Main description:

This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

Contents:

1. The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications

Natalie S. Poulter, William T.E. Pitkeathly, Philip J. Smith, and Joshua Z. Rappoport

2. Two-Photon Excitation Microscopy And Its Applications In Neuroscience

Ricardo Mostany, Amaya Miquelajauregui, Matthew Shtrahman, and Carlos Portera-Cailliau

3. Live Spheroid Formation Recorded with Light Sheet-based Fluorescence MicroscopyFrancesco Pampaloni, Roli Richa, Nariman Ansari, and Ernst H.K. Stelzer

4. Fluorescence Microscopy Based RNA Interference Screening

Manuel Gunkel, Nina Beil, Jurgen Beneke, Jurgen Reymann, and Holger Erfle

5. Fluorescence Resonance Energy Transfer Microscopy (FRET)

Kasia M. Kedziora and Kees Jalink

6. Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy

Yuansheng Sun, Vinod Jyothikumar, and Ammasi Periasamy

7. Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation

Bart Geverts, Martin E. van Royen, and Adriaan B. Houtsmuller

8. Fluorescence Correlation Spectroscopy

Mark A. Hink

9. Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules

Suvrajit Saha, Riya Raghupathy, and Satyajit Mayor

10. Practical Structured-Illumination Microscopy E. Hesper Rego and Lin Shao

11. 4Pi Microscopy of the Nuclear Pore Complex

Martin Kahms, Jana Huve, and Reiner Peters

12. Application Of STED Microscopy To Cell Biology Questions

Natalia H. Revelo and Silvio O. Rizzoli

13. Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes

Kelsey Temprine, Andrew G. York, and Hari Shroff

14. Direct Stochastic Optical Reconstruction Microscopy (dSTORM)

Ulrike Endesfelder and Mike Heilemann

15. Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells

Taofei Yin and Yi I. Wu