Main description:

Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. The international panel of authors also provide animal models and numerous studies concentrating on mitochondria during hypoxic conditions using advanced methods for pO2, peroxynitrate, reactive S-nitrosothiols, lipid peroxides, COX, and the mitochondrial membrane potential. Due to the dynamic nature of this topic, this book is the second of several volumes of Advanced Protocols in Oxidative Stress, all included in the highly successful Methods in Molecular Biology (TM) series. As part of the series, the chapters of this volume present brief introductions to the respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting to ensure easy replication of the technology involved.

Authoritative and convenient, Advanced Protocols in Oxidative Stress II is an ideal desk reference for scientists wishing to further the research in this exciting, unique, and vital field of study.

Contents:

Part I: Reactive Oxygen and Nitrogen Techniques

1. Current Status of Measuring Oxidative Stress
Beniamino Palmieri and Valeriana Sblendorio

2. PO2 and ROS/RNS Measurements in the Microcirculation in Hypoxia
Silvia Bertuglia and Marcos Intaglietta

3. Nitrate Reductase Activity of Mitochondrial Aldehyde Dehydrogenase (ALDH-2) as a Redox Sensor for Cardiovascular Oxidative Stress
Andreas Daiber and Thomas Munzel

4. Identification of ROS Using Oxidized DCFDA and Flow-Cytometry
Evgeniy Eruslanov and Sergei Kusmartsev

5. Measurement of 8-Isoprostane in Exhaled Breath Condensate
Paolo Montuschi, Peter J. Barnes, and Giovanni Ciabattoni

6. Electron Paramagnetic Resonance Oximetry and Redoximetry
Guanglong He

7. Measurement of Mitochondrial Membrane Potential and Proton Leak
Gaetano Serviddio and Juan Sastre

8. Determination of Erythrocyte Fragility as a Marker of Pesticide-Induced Membrane Oxidative Damage
Bechan Sharma, Devendra K. Rai, Prashant Kumar Rai, S.I. Rizvi, and Geeta Watal

9. Using N, N, N', N'-Tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro
Nenad Petrovic and Michael Murray

10. Structural and Functional Changes in the Insulin Molecule Produced by Oxidative Stress
Rafael Medina-Navarro, Alberto M. Guzman-Grenfell, Ivonne Olivares-Corichi, and Juan J. Hicks

11. Multiphoton Redox Ratio Imaging for Metabolic Monitoring in vivo
Melissa Skala and Nirmala Ramanujam

12. Using Fluorescence-Activated Flow Cytometry to Determine Reactive Oxygen Species Formation and Membrane Lipid Peroxidation in Viable Boar Spermatozoa
H. David Guthrie and Glenn R. Welch

13. Lipofuscin: Detection and Quantification by Microscopic Techniques
Tobias Jung,Annika Hoehn, and Tilman Grune

Part II: Antioxidant Technology and Application

14. Oxy-Score: A Global Index to Improve Evaluation of Oxidative Stress by Combining Pro- and Antioxidant Markers
Fabrizio Veglia, Viviana Cavalca, and Elena Tremoli

15. CUPRAC (Cupric Ion Reducing Antioxidant Capacity) Assay for Antioxidants in Human Serum and for Hydroxyl Radical Scavengers
Resat Apak, Kubilay Guclu, Mustafa OEzyurek, Burcu Bektasoglu, and Mustafa Bener

16. Analysis of Antioxidant Activities in Vegetable Oils and Fat Soluble Vitamins and Biofactors by the PAO-SO Method
Kazuo Sakai, Satoko Kino, Masao Takeuchi, Tairin Ochi, Giuseppe Da Cruz, and Isao Tomita

17. Measuring Antioxidant Capacity Using the ORAC and TOSC Assays
Andrew R. Garrett, Byron K. Murray, Richard A. Robison, and Kim L. O'Neill

18. Assessing the Neuroprotective Effect of Antioxidant Food Factors by Application of Lipid-Derived Dopamine Modification Adducts
Xuebo Liu, Naruomi Yamada, and Toshihiko Osawa

19. LIBS-Based Detection of Antioxidant Elements: A New Strategy
Geeta Watal, Bechan Sharma, Prashant Kumar Rai, Dolly Jaiswal, Devendra K. Rai, Nilesh K. Rai, and A.K. Rai

20. A Method for Evaluation of Antioxidant Activity Based on Inhibition of Free Radical-Induced Erythrocyte Hemolysis
Jun Takebayashi, Jianbin Chen, and Akihiro Tai

21. Design and Synthesis of Antioxidant a-Lipoic Acid Hybrids
Maria Koufaki and Anastasia Detsi

22. Characterization of the Antioxidant Properties of Pentaerithrityl Tetranitrate (PETN)-Induction of the Intrinsic Antioxidative System Heme Oxygenase-1 (HO-1)
Andreas Daiber and Thomas Munzel

23. Direct Determination of Tissue Aminothiol, Disulfide, and Thioether Levels Using HPLC-ECD with a Novel Stable Boron-Doped