Main description:

This book addresses the design of emerging conceptual tools, technologies and systems including novel synthetic parts, devices, circuits, oscillators, biological gates, and small regulatory RNAs (riboregulators and riboswitches), which serve as versatile control elements for regulating gene expression. Synthetic biology, a rapidly growing field that involves the application of engineering principles in biology, is now being used to develop novel systems for a wide range of applications including diagnostics, cell reprogramming, therapeutics, enzymes, vaccines, biomaterials, biofuels, fine chemicals and many more.

The book subsequently summarizes recent developments in technologies for assembling synthetic genomes, minimal genomes, synthetic biology toolboxes, CRISPR-Cas systems, cell-free protein synthesis systems and microfluidics. Accordingly, it offers a valuable resource not only for beginners in synthetic biology, but also for researchers, students, scientists, clinicians, stakeholders and policymakers interested in the potential held by synthetic biology.

Contents:

Chapter 1. Fundamentals of Synthetic Biology.- Chapter 1.1 Introduction to synthetic biology.- Chapter 1.2. Current progress in synthetic genetic networks- Chapter 1.3. Current progress in design and construction of synthetic parts, devices and systems.- Chapter 1.4. Current status and challenges of DNA sequencing.- Chapter 1.5. Current progress in DNA synthesis technology.- Chapter 1.6. Recent development of synthetic biology toolbox.- Chapter 1.7. Recent development of automaton computing machine.- Chapter 1.8. Biomimetic approaches in synthetic biology.- Chapter 2. Applied Synthetic Biology.- Chapter 2.1. Recent advances and use of the synthetic genetic oscillators.- Chapter 2.2. Recent development and applications of synthetic of biological gates.- Chapter 2.3. Engineering of riboregulators for gene regulation.- Chapter 2.4. Design and construction of riboswitches.- Chapter 2.5. Recent advances in gene and genome assembly.- Chapter 2.6. Recent development of synthetic genomics.- Chapter 2.7. Minimal genome and synthetic cell.- Chapter 2.8. Expansion of genetic code.- Chapter 2.9. Expanding the potential of CRISPR-Cas9 technology.- Chapter 2.10. Development of cell free synthetic biology.- Chapter 2.11. Application and challenges of synthetic biology.- Chapter 2.12. Development and application of microfluidics in synthetic biology.- Chapter 2.13. Ethical, patents and regulatory issues.