Main description:

This book provides a comprehensive overview of the major biochemical analytical techniques, with detailed descriptions of the instrumentation and applications. The contributions, which each focus on a specific technique, are based on a thorough review and analysis of the current literature as well as the authors' experiences in the lab.

Divided into nine parts, the book provides insights into basic separation techniques like sedimentation, filtration and centrifugation, as well as analytical techniques such as spectrophotometry, chromatography, electrophoresis, immuno-techniques, radioactivity and microscopy.

Contents:

1 Sedimentation 1.1 Gravity sedimentation 1.2 Zone Settling &Compression2 Filtration 2.1 Microfiltration 2.2 Ultrafiltration3 Centrifugation 3.1 Two basic modes of centrifugation 3.2 Instrument Design4 Colorimetry and spectrophotometer (spectrophotometry) 4.1 Colorimetry 4.2 Spectrophotometry5 Chromatography 5.1 Partition Chromatography 5.1.1 Paper chromatography 5.1.2 Thin Layer Chromatography 5.1.3 HPLC 5.1.4 Gas Chromatography 5.1.5 Gel filtration chromatography6 Electrophoresis
6.1 Paper Electrophoresis
6.2 SDS-Polyacrylamide Gel Electrophoresis
6.3 Immunoelectrophoresis
7 Immuno-techniques
7.1 Immunodiffusion
7.2 Immuno-fluorescence
7.3 Immunoassay: Enzyme Linked Immunosorbent Assay (ELISA)
7.4 Radioimmunoassay (RIA)
7.5 Western blotting or Immunoblotting
7.6 Southern Blotting
7.7 Northern Blot
8. Radioactivity
9. Microscopy