Main description:

In
addition to updating important core techniques this third edition presents new
chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and
pericyte isolation techniques, spheroid and arterial ring based in vitro
assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols,
Third Edition also guides readers through new protocols on chorioallantoic
membrane models, corneal pocket assays to assess angio- and lymphangiogenesis,
models of muscle angiogenesis, and use of zebrafish embryos to study vascular
angiogenesis and senescence. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.

Authoritative
and practical, Angiogenesis Protocols, Third Edition provides not only a
practical handbook for key techniques, but also an informative and enjoyable
read for all those interested, no matter how directly, in angiogenesis.

Contents:

Part I: Microscopic Assessment

1. Assessing Tumor
Angiogenesis in Histological Samples

Jia-Min Pang, Nicholas Jene, and Stephen B Fox

2. Immunohistochemical Methods
for Measuring Tissue Lymphangiogenesis

Daniel J. Royston, Steven Clasper, and David G. Jackson

3. Immunohistochemical Assessment
of Leukocyte Involvement in Angiogenesis

Narmeen S. Ahmad, Stewart G. Martin, and Sarah J. Storr

Part II: Cell Isolation Techniques

4. Isolation and Culture of
Human Endothelial Cells from Micro- And Macro-Vessels

Peter W Hewett

5. Isolation,
Identification and Culture of Human Lymphatic Endothelial Cells

Zerina Lokmic

6. Isolation, Culture and
Characterisation of Vascular Smooth Muscle Cells

Jessal J Patel and Richard CM Siow

7. Isolation and Transfection
of Primary Culture Bovine Retinal Pericytes

Vincent A. Primo and Joseph F. Arboleda-Velasquez

Part III: In Vitro Techniques

8. In Vitro Assays for Endothelial Cell
Functions Required for Angiogenesis: Proliferation, Motility, Tubular
Differentiation And Matrix Proteolysis

Suzanne A Eccles, William Court, and Lisa Patterson

9. Tube Forming Assays

Ryan M. Brown, Christopher J. Meah, Victoria L. Heath, Iain B. Styles,
and Roy Bicknell

10. In Vitro Co-Culture
Assays of Angiogenesis

Mark Richards and Harry Mellor

11. Spheroid-Based In Vitro
Angiogenesis Model

Larissa Pfisterer and Thomas Korff

12. Stem Cell Spheroid-Based Sprout Assay in
Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study Of
Angiogenesis

Fatemeh Sharifpanah and Heinrich Sauer

13. Human Arterial Ring
Angiogenesis Assay

Giorgio Seano and Luca Primo

14. A Modified Aortic Ring
Assay to Assess Angiogenic Potential In Vitro

Nina Zippel, Yindi Ding, and Ingrid Fleming

15. Quantitative Imaging-Based Examination of
Pericyte Control of Endothelial Growth Dynamics and Angiogenesis.

Anthony R. Sheets, Jennifer T. Durham, and Ira M. Herman

16. Static and Dynamic Assays
of Cell Adhesion Relevant to the Vasculature

Lynn M. Butler, Helen M. Mcgettrick, and Gerard B. Nash

Part IV: In Vivo Techniques

17. Dorsal Skinfold Chamber Preparation in
Mice: Studying Angiogenesis by Intravital Microscopy

Axel Sckell and Michael Leunig

18. Quantitative Estimation of
Tissue Blood Flow Rate

Gillian M Tozer, Vivien E Prise, and Vincent J Cunningham

19. Chorioallantoic Membrane Microtumor Model
to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor
Cell Intravasation

Elena I. Deryugina

20. The Rabbit Corneal Pocket
Assay

Lucia Morbidelli and Marina Ziche

21. The Corneal Micropocket
Assay: A Model of Angiogenesis and
Lymphangiogenesis

Shintaro Nakao and Ali Hafezi-Moghadam

22. Models of Oxygen Induced
Retinopathy in Rodents

Melissa V. Gammons and David O. Bates

23. The Sponge Implant Model of
Angiogenesis

Silvia Passos Andrade and Monica Alves Neves Diniz Ferreira

24. Measurement of Angiogenesis,
Arteriolargenesis and Lymphangiogenesis Phentotypes by Use of Two-Dimensional
Mesenteric Angiogenesis Assay

Andrew V. Benest and David O. Bates

25. In Vivo Models of Muscle
Angiogenesis

Stuart Egginton

26. Use of the Hollow Fibre Assay to Evaluate
Agents that Target the Tumor Neovasculature

Patricia A. Cooper
and Steven D. Shnyder

27. Studying Vascular Angiogenesis and
Senescence in Zebrafish Embryos

Emma Ristori, Sandra Donnini, and Marina Ziche