Main description:

The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production.

The chapters are written by world-renowned experts and the volume's five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.

Contents:

Animal Cell Biotechnology - Methods and Protocols

Editor: Ralf Poertner

Contents

Preface

Contributors

Part I. Basic techniques for establishing production cell lines


1 Transduction Technologies
David A. Fluri and Martin Fussenegger

2 Cells for gene therapy and vector production
C. Delenda, M. Chillon, A.-M. Douar and Otto-Wilhelm Merten

3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
Alexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier

4 DNA Fingerprinting and Characterisation of Animal Cell Lines
Glyn N. Stacey, Ed Byrne and J. Ross Hawkins

Part II. Basic cultivation techniques

5 Microcarrier Cell culture Technology
Gerald Bluml

6 Cell Encapsulation
Holger Hubner

7 Tools for High-Throughput Medium and Process Optimisation
Martin Jordan and Nigel Jenkins


Part III. Cell characterisation and analysis

8 Cell counting and viability measurements
Michael Butler and Maureen Spearman

9 Monitoring of Growth, Physiology and Productivity of animal cells by Flow Cytometry
Silvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai

10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
Andre A. Neves and Kevin M. Brindle

11 Methods for off-line analysis of nutrients and products in mammalian cell culture
Heino Buntemeyer

12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation
Derek Adams, Rashmi Korke, and Wei-Shou Hu

13 Measurement of apoptosis in cell culture
Adiba Ishaque1 and Mohamed Al-Rubeai

14 Metabolic Flux Estimation in Mammalian Cell Cultures
Chetan T. Goudar, Richard Biener, JamesM. Piret and Konstantin B. Konstantinov


Part IV. Special cultivation techniques

15 Disposable bioreactors for inoculum production and protein expression
Regine Eibl and Dieter Eibl

16 Hollow Fibre Cell Culture
John M. Davis

17 Cultivation of mammalian cells in fixed bed reactors
Ralf Poertner and Oscar B.J. Platas Barradas

18 Configuration of Bioreactors
Dirk E. Martens and Evert Jan van den End




Part V. Downstream techniques

19 Membrane filtration in animal cell culture
Peter Czermak, Dirk Nehring and Ranil Wickramasinghe

20 Chromatographic techniques in the downstream processing of (recombinant) proteins
Ruth Freitag

Part VI. Special applications

21 Vaccine production - state of the art and future needs in upstream processing
Yvonne Genzel and Udo Reichl

22 Retrovirus production and characterization
Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues, Paula Alves

23 Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production
Sabine Geisse

Index