Main description:

This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

Contents:

Part I: Introduction

1. Animal Coronaviruses: A Brief Introduction

Leyi Wang and Yan Zhang

Part II: Conventional Technologies

2. Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues

Kwonil Jung

3. Virus Neutralization Assay for Turkey Coronavirus Infection

Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin

4. Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli

Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin

5. Antibody-Capture Enzyme-Linked Im

munosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen

Chien Chang Loa, Mohamed Abdelwahab, Yi-Ning Chen, Ming-Kun Hsieh, Ching Ching Wu, and Tsang Long Lin

Part III: Reverse Genetics

6. Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus

Sarah M. Keep, Erica Bickerton, and Paul Britton

7. Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses

Gergely Tekes

Part IV: Molecular Diagnostics

8. Real-Time RT-PCR Detection of Equine Coronavirus

Fabien Miszczak, Nathalie Kin, Vincent Tesson, and

Astrid Vabret

9. Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction

Amauri Alcindo Alfieri, Alice Fernandes Alfieri, and Elisabete Takiuchi

10. Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus

Ramesh Vemulapalli

11. An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes

Junfeng Sun and Shengwang Liu

12. A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus

Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin

13. Real-Time Reverse

Transcription-polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues

Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin

14. PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene

Yi-Ning Chen, Aydemir Akin, Chien Chang Loa, Mustafa Ababneh, Jianzhong Cao, Wan-Jung Chen, Ching Ching Wu, and Tsang Long Lin

15. Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis

Takehisa Soma

16. Genotype-Specific Detection of Ferret Coronavirus by Conventional and Real-Time Reverse Transcription Polymerase Chain Reaction

Annabel G. Wise, Matti Kiupel, and Roger K. Maes

17. Molecular Charac

terization of Canine Coronavirus

Rita de Cassia Nasser Cubel Garcia

Part V: Next Generation Sequencing

18. Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform

Leyi Wang, Tod Stuber, Patrick Camp, Suelee Robbe-Austerman, and Yan Zhang

19. Next Generation Sequencing for Porcine Coronaviruses

Douglas Marthaler, Ann Bohac, Aaron Becker, and Nichole Peterson