Main description:

This detailed volume provides a single, valuable reference source for
methods that definitively identify and accurately quantify apoptosis. The book
begins with common methods utilized to detect and quantitate apoptosis, as well
as apoptosis signaling pathways in toxicological and other related research. It
continues with multi-parametric and phased apoptosis assays for detecting early
and late apoptosis or distinguishing apoptosis from necrosis and autophagy.
Subsequent chapters focus on recent advances in real time and high-throughput
assays that detect and quantitate apoptosis and apoptosis signaling pathways.
Final chapters focus on recent developments in preclinical anticancer
therapeutics targeting apoptosis. Written for the Methods in Pharmacology
and Toxicology series, chapters feature step-by-step descriptions of the
methodologies, as well as expert tips and implementation advice.

Vital and authoritative, Apoptosis Methods in
Toxicology serves novice scientists as well as experts,
utilizing a range of instruments from common laboratory equipment to high-end
expensive and automated machinery capable of performing real time apoptotic
measurements.

Contents:

1. An Overview of
Apoptosis Methods in Toxicological Research: Recent Updates

Perpetua M.
Muganda

2. Detection of Apoptosis: From Bench Side
to Clinical Practice

William P.
Bozza, Julianne D. Twomey, Su-Ryun Kim, and Baolin Zhang

3. Determining the
Extent of Toxicant-Induced Apoptosis Using Concurrent Phased Apoptosis Assays

Akamu Jude
Ewunkem and Perpetua M. Muganda

4. Measurement of Apoptosis
by Multiparametric Flow Cytometry

William
Telford

5. Detecting
Apoptosis, Autophagy, and Necrosis

Jack
Coleman, Rui Liu, Kathy Wang, and Arun Kumar

6. A Low-Cost Method
for Tracking the Induction of Apoptosis Using FRET-Based Activity Sensors in
Suspension Cells

Akamu J.
Ewunkem, Carl D. Parson II, Perpetua M. Muganda, and Robert H. Newman

7. FRET-Based
Measurement of Apoptotic Caspase Activities by High-Throughput Screening Flow
Cytometry

Christian T. Hellwig, Agnieszka H.
Ludwig-Galezowska, and Markus Rehm

8. Automated
Ratio Imaging Using Nuclear-Targeted Fret Probe-Expressing Cells for Apoptosis
Detection

Krupa Ann
Mathew, Deepa Indira, Jeena Joseph, Prakash Rajappan Pillai, Indu Ramachandran,
Shankara Narayanan Varadarajan, and Santhoshkumar Thankayyan Retnabai

9. Antibody-Based Proteomic Analysis of
Apoptosis Signaling

Matthew P. Stokes, Hongbo Gu, and
Jeffrey C. Silva

<10. Novel Electrochemical Biosensor for
Apoptosis Evaluation

Peng Miao and Jian Yin

11. Microplate-Based Whole Zebrafish Caspase 3/7 Assay
for Screening Small Molecule Compounds

Wen Lin Seng, Dawei Zhang, and
Patricia McGrath

12. Targeting Cancer Cell Death with Small-Molecule Agents
for Potential Therapeutics

Lan Zhang, Yaxin Zheng, Mao Tian,
Shou-yue Zhang, Bo Liu, and Jinhui Wang

13. Liposomes in Apoptosis Induction and Cancer Therapy

Magisetty Obulesu and
Magisetty Jhansilakshmi