Main description:

The earliest descriptions of human chromosomes initiated the genomics revolution that is now upon us. Array Comparative Genomic Hybridization: Protocols and Applications explores the scope of what is now possible as far as documenting abnormalities associated with several human cancers. While the technology for interrogating the human genome continues to evolve, refinement of comparative genomic hybridization (CGH) using array CGH and related technologies have provided enormous insight into human cancers at an affordable scale. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Array Comparative Genomic Hybridization: Protocols and Applications provides researchers with well-honed methodologies to learn these techniques for their own use in research or clinical diagnostic laboratories.

Contents:

1. Array Comparative Genomic Hybridization: An Overview of Protocols, Applications and Technology Trends

Diponkar Banerjee

2. Ultra Dense Array CGH and Discovery of Micro-Copy Number Alterations and Gene Fusions in the Cancer Genome

Ewa Przybytkowski, Adriana Aguilar-Mahecha, Sheida Nabavi, Peter J. Tonellato, and Mark Basik

3. Epigenomics: Sequencing the Methylome

Martin Hirst

4. Application of Array Comparative Genomic Hybridization in Chronic Myeloid Leukemia

Seonyang Park, Youngil Koh, Seung-Hyun Jung, and Yeun-Jun Chung

5. The Use of Cytogenetic Microarrays in Myelodysplastic Syndrome Characterization

Lisa G. Shaffer, Blake C. Ballif, and Roger A. Schultz

6. CGH Protocols - Chronic Lymphocytic Leukemia

Esteban Braggio, Rafael Fonseca, and Neil E. Kay

7. Analysis of Acquire Genomic Copy Number Aberrations and Regions of Loss of Heterozygosity in Acute Myelogenous Leukemia Fenomes using Affymetrix SNP 6.0 Arrays and Supporting Software Tools

Peter D. Ouillete, Kerby A. Shedden, Cheng Li, and Sami N. Malek

8. Clinical Applications of BAC Array-CGH to the Study of Diffuse Large B-Cell Lymphomas

Cristina Robledo, Juan Luis Garcia, and Jesus M. Hernandez

9. Genomic Profiling of Mantle Cell Lymphoma

Melissa Rieger and Jose A. Martinez-Climent

10. Copy Number Analysis in EBV-Positive Nodal Peripheral T-Cell Lymphoma, Unspecified

Kyusam Choi, Hyunjeong Ju, and Young Hyeh Ko

11. Mycosis Fungoides and Sezary Syndrome

Blanca Espinet and Rocio Salgado

12. Array CGH Reveals Clonal Evolution of Adult T Cell Leukemia/Lymphoma

Akira Umino and Masao Seto

13. Array-CGH Analysis of Cutaneous Anaplastic Large Cell Lymphoma

K. Szuhai, R. Van Doorn, and C.P. Tensen

14. Genome Wide DNA-Profiling of HIV-Related B-Cell Lymphomas

Andrea Rinaldi, Daniela Capello, Emanuele Zucca, Gianluca Gaidano, and Franceso Bertoni

15. Array Comparative Genomic Hybridization in Osteosarcoma

Bekim Sadijovi, Paul C. Park, Shamini Selvarajah, and Maria Zielenska

16. Comparative Genomic Hybridization of Wilms' Tumor

Shahrad Rod Rassekh and Evica Rajcan-Separovic

17. Array-CGH in Childhood MDS

Marcel Tauscher, Inka Praulich, and Doris Steinemann

18. Non-Familial Breast Cancer Subtypes

Markus Ringner, Johan Staaf, and Goeran Joensson

19. Applications of Array-CGH for Lung Cancer

Kenneth J. Craddock, Wan L. Lam, and Ming-Sound Tsao

20. Array CGH in Brain Tumours

Gayatry Mohapatra, Julia Sharma, and Stephen Yip

21. Finding Common Regions of Alteration in Copy Number Data

Oscar M. Rueda, Ramon Diaz-Uriarte, and Carlos Caldas

22. Distinguishing Somatic and Germline Copy Number Events in Cancer Patient DNA Hybridized to Whole Genome SNP Genotyping Arrays

Gavin Ha and Sohrab Shah