Main description:

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.

Contents:

Part I: Introduction

1. A Historical Perspective on Bacterial Persistence
Natalie Verstraeten, Wouter Knapen, Maarten Fauvart, Jan Michiels

Part II: Quantification of Persistence

2. Persisters-Methods for Isolation and Identifying Contributing Factors: A Review
Sarah E. Rowe, Brian P. Conlon, Iris Keren, and Kim Lewis

3. A General Method for Measuring Persister Levels in Escherichia Coli Cultures
Niilo Kaldalu, Arvi Joers, Henri Ingelman, and Tanel Tenson

4. Optimized Method for Measuring Persistence in Escherichia cCli with Improved Reproducibility
F. Goormaghtigh and L. Van Melderen

5. A Microplate-Based System as In Vitro Model of Biofilm Growth and Quantification
Ilse Vandecandelaere, Heleen Van Acker, and Tom Coenye

6. Protocol for Determination of the Persister Subpopulation in Candida Albicans Biofilms
Katrijn De Brucker, Kaat De Cremer, Bruno P.A. Cammue, and Karin Thevissen

Part III: Single Cell Analysis of Persister Cells

7. Quantitative Measurements of Type I and Type II Persisters Using ScanLag
Irit Levin-Reisman and Nathalie Q. Balaban

8. Analyzing Persister Physiology with Fluorescence-Activated Cell Sorting
Mehmet A. Orman, Theresa C. Henry, Christina J. DeCoste, and Mark P. Brynildsen

9. Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array
Ryota Iino, Shouichi Sakakihara, Yoshimi Matsumoto, and Kunihiko Nishino

Part IV: Identification of Persister Mutants and Genes

10. A Whole-Cell Based High-Throughput Screening Method to Identify Molecules Targeting Pseudomonas Aeruginosa Persister Cells
Veerle Liebens, Valerie Defraine, and Maarten Fauvart

11. Functional Analysis of the Role of Toxin-Antitoxin (TA)

Loci in Bacterial Persistence
Aaron T. Butt and Richard W. Titball

12. Experimental Evolution of Escherichia Coli Persister Levels Using Cyclic Antibiotic Treatments
Bram Van den Bergh, Joran E. Michiels, and Jan Michiels

Part V: Cellular and Animal Model Systems for Studying Persistence

13. In Vitro Models for the Study of the Intracellular Activity of Antibiotics
Julien M. Buyck, Sandrine Lemaire, Cristina Seral, Ahalieyah Anantharajah, Frederic Peyrusson, Paul M. Tulkens, and F. Van Bambeke

14. A Murine Model for Escherichia Coli Urinary Tract Infection
Thomas J. Hannan and David A. Hunstad

15. Analysis of Macrophage-Induced Salmonella Persisters
Robert A. Fisher, Angela M. Cheverton, and Sophie Helaine

16. Population Dynamics Analysis of Ciprofloxacin-Persistent S. Typhimurium Cells in a Mouse Model for Salmonella Diarrhea
Patrick Kaiser, Roland R. Regoes, and Wolf-Dietrich Hardt

Part VI: Mathematical Modeling of Persistence

17. Computational Methods to Model Persistence
Alexandra Vandervelde, Remy Loris, Jan Danckaert, and Lendert Gelens