Main description:

This text is a review of molecular immunohematology (MI). It draws from analyses and case studies around the world and details many techniques used in many labs. It is aimed at anyone interested in how MI is changing blood bank and transfusion medicine.

Contents:

Chapter 1: An overview of the Classical Serological methods: limitations and benefits of serology and DNA testing
John J. Moulds

Chapter 2: Introduction to Molecular Typing
Joann M. Moulds, Steven R. Sloan*

Chapter 3: The BeadChip System: A Flexible Array Format for Complex Nucleic Acid and Protein Analysis
Ghazala Hashmi*, Yi Zhang, Michael Seul

Chapter 4: Implementation of HEA at Blood Centers: Prescreening, Rare Donors, Inventory Management
Joann M. Moulds

Chapter 5: Implementation of HEA BeadChip System at Medical Centers: Providing Extended Matched Units and Eliminating Complex Workups for Patients
Ellen Klapper

Chapter 6: Human Platelet Antigen Genotyping and Diagnosis of Antiplatelet Alloimmunization
Gerald Bertrand, Cecile Kaplan-Gouet*

Chapter 7: Blood group genotyping by high-throughput DNA-analysis: Application to the Panel National de Reference du CNRGS
Sandrine Kappler-Gratias, Thierry Peyrard, Pierre Yves Le Pennec, Jean-Pierre Cartron, Philippe Rouger, Bach-Nga Pham*

Chapter 8: Implementation and Assessment of High-Throughput Donor Typing at the Milan, Italy Immunohematology Reference Laboratory
Nicoletta Revelli*, Cinzia Paccapelo, Paola Ponzo, Francesca Truglio, Veronica Sala, Francesca Poli, Maurizio Marconi, Maria Antonietta Villa

Chapter 9: Implementation of the BioArray Erythrocyte Antigen (HEA) genotyping System BeadChip (TM) HEA: The Red Cross of Spain Experience
Luisa Barea Garcia, Marta Martinez Sanchez, Gemma Cabrera Rodriguez-Escalona, Emma Castro Izaguirre*

Chapter 10: Looking Beyond HEA: Matching SCD Patients for RH Variants
Marion E Reid*, Christine Halter Hipsky

Chapter 11: Identification of Altered RHD and RHCE Alleles: A Comparison of Manual and Automated Molecular Methods
Sunitha Vege, Connie M. Westhoff*

Chapter 12: Bayesian Classification Algorithms for Automated Allele Assignment
Kairali Podual, Yi Zhang, Natalia Mezokh, JiaCheng Yang, Randall Wilson*