Main description:

BetaSys uses the example of regulated exocytosis in pancreatic -cells, and its relevance to diabetes, to illustrate the major concepts of systems biology, its methods and applications.

Contents:

Preface.- Systems biology of -cells - revisited.- Established facts and open questions of the focused systems analysis of regulated exocytosis in -cells - a background for a focused systems analysis approach.- Mitochondria and metabolic signals in -cells.- -cell ontogenesis and the insulin production apparatus.- The role of the cytoskeleton in transport and release of the insulin-containing granules by pancreatic -cells.- The mathematical microscope making the inaccessible accessible.- Magnetic resonance imaging of pancreatic -cells.- Mapping the -cell in 3D at the nanoscale using novel cellular electron tomography and computational approaches.- In vivo applications of inorganic nanoparticles.- Cell cultivation and sensor-based assays for dynamic measurements of cell vitality: basic concepts.- Bioimpedance spectroscopy.- DNA variations, impaired insulin secretion and type-2 diabetes.- Genetically programmed defects in -cell function.- Proteomic analysis of the pancreatic islet -cell secretory granule: current understanding and future opportunities.- Physiological and pathophysiological role of islet amyloid polypeptide (IAPP, amylin) - a role in the pathogenesis of type-2 diabetes.- Present state of islet transplantation for type-1 diabetes patients.- Predictive protein networks and identification of drugable targets in the -cell.- Nanotoxicity.- From silicon cell to silicon human.- Probing cellular dynamics with mesoscopic simulations.- What drives calcium oscillations in -cells? new tasks for cyclic analysis.- Whole-body and cellular models of glucose-stimulated insulin secretion.- Geometric and electromagnetic aspects of fusion pore making.- Index.