Main description:

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps.

Key Features:

Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience

Includes chemical biology as its foundation, teaching readers experimental methods specific to the field

Provides instructor experiments that are easy to prepare and execute, at comparatively low cost

Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry

Is written by an expert in the field

This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.

Contents:

Author Bio

Chapter 1 Buffers

Chapter 2 Assays

Chapter 3 Protein Concentration

Chapter 4 ELISA

Chapter 5 Salting Out Proteins and Other Biomolecules

Chapter 6 A Discussion of Isoelectric Point and Effective Charge

Chapter 7 Column Chromatography

Chapter 8 Michaelis-Menten Kinetics

Chapter 9 Protein Purification

Chapter 10 Polyacrylamide Gels

Chapter 11 In Silico Biochemistry: The Evolution of Globins

Chapter 12 Growing Crystals of Hemoglobin

Chapter 13 Enzyme Inhibition

Chapter 14 Multisubstrate Kinetics

Chapter 15 Fluorescence and Denaturation

Chapter 16 Fluorescence Studies of Ligand Binding

Chapter 17 DNA Restriction Digests

Chapter 18 Western Blotting

Index