Main description:

This fully updated edition provides the most recent advances in cancer cell signaling knowledge combined with some discussion of the current challenges and prospects in cancer therapy. Beginning with an important section on cancer resistance, the main obstacle to effective cancer therapy, the book continues with chapters exploring state-of-the-art methods in epigenetic control of cancer, metastasis promotion, as well as a variety of new technologies in the study of cancer. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Third Edition serves as an ideal guide to researchers seeking to overcome the challenges in the vital field of cancer cell signaling.

Contents:

Part I: Cancer Resistance

1. Fighting Cancer Resistance: An Overview

Martha Robles-Flores

2. Prostate Cancer Spheroids: A Three-Dimensional Model for Studying Tumor Heterogeneity

Mauricio Rodriguez-Dorantes, Carlos David Cruz-Hernandez, Sergio Alberto Cortes-Ramirez, Jenie Marian Cruz-Burgos, Juan Pablo Reyes-Grajeda, Oscar Peralta-Zaragoza, and Alberto Losada-Garcia

3. Enrichment and Transcriptional Characterization of Stem Cells Isolated from Human Glioblastoma Cell Lines

Ana G. Pina-Medina, Ana M. Hernandez-Vega, Nestor F. Diaz, Ismael Mancilla-Herrera, and Ignacio Camacho-Arroyo

4. Reverse Docking for the Identification of Molecular Targets of Anticancer Compounds

Angel Jonathan Ruiz-Moreno, Alexander Doemling, and Marco Antonio Velasco-Velazquez

5. Mouse Model for Efficient Simultaneous Targeting of Glycolysis, Glutaminolysis, and De Novo Synthesis of Fatty Acids in Colon Cancer

Alejandro Schcolnik-Cabrera and Alfonso Duenas-Gonzalez

Part II: Epigenetic Control of Cancer

6. Developing a Portable Device for the Identification of miRNAs in Fluids

Alexander Asanov, Alicia Sampieri, and Luis Vaca

7. Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling

Yi Feng, Junjie Jiang, Zhongyi Hu, Jiao Yuan, Tianli Zhang, Yutian Pan, Mu Xu, Chunsheng Li, Youyou Zhang, Lin Zhang, and Xiaowen Hu

8. RNA-Sequencing Analysis Pipeline for Prognostic Marker Identification in Cancer

Sudhanshu Shukla and Seema Khadirnaikar

Part III: Metastasis Promotion

9. Correlation of Circulating Tumor Cell Measurements with 3D Quantitative Tumor Characterization to Predict Clinical Outcomes in Cancer

Sweet Ping Ng, Clifton David Fuller, and Heath Devin Skinner

10. Extracellular Vesicles and Their Roles in Cancer Progression

Wen-Hsuan Chang, Richard A. Cerione, and Marc A. Antonyak

11. Analysis of Tumor-Derived Exosomes by Nanoscale Flow Cytometry

Cynthia Lopez-Pacheco, Andrea Bedoya-Lopez, Roxana Olguin-Alor, and Gloria Soldevila

12. In Vitro Models for Studying Tumor Progression

Juan Carlos Gonzalez-Orozco, Saul Gaona-Dominguez, and Ignacio Camacho-Arroyo

13. Assessment of Cell Cycle in Primitive Chronic Myeloid Leukemia Cells by Flow Cytometry after Co-Culture with Endothelial Cells

Patricia Torres-Barrera, Mireya Ramirez-Florencio, and Antonieta Chavez-Gonzalez

Part IV: New Technologies in the Study of Cancer

14. Chimeric Antigen Receptor (CAR) T-Cell Therapy for Cancer: Challenges and Opportunities

Arimelek Cortes-Hernandez, Evelyn Alvarez-Salazar, and Gloria Soldevila

15. Cell-Internalization SELEX of RNA Aptamers as a Starting Point for Prostate Cancer Research

Mauricio Rodriguez-Dorantes, Sergio Alberto Cortes-Ramirez, Jenie Marian Cruz-Burgos, Juan Pablo Reyes-Grajeda, Alberto Losada-Garcia, Vanessa Gonzalez-Covarrubias, and Carlos David Cruz-Hernandez

16. Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9

Francisco Carmona-Aldana, Hober N Nunez-Martinez, Carlos A Peralta-Alvarez, Gustavo Tapia-Urzua, and Felix Recillas-Targa

17. Developing a Model for a siRNA Delivery System by Cancer Implantation into Zebrafish Circulation

Yasuhito Shimada and Mai Hazekawa

18. Fabrication of Adhesive Substrate for Incorporating Hydrogels to Investigate the Influence of Stiffness on Cancer Cell Behavior

Genaro Vazquez-Victorio, Adriana Rodriguez-Hernandez, Mariel Cano-Jorge, Ana Ximena Monroy-Romero, Marina Macias-Silva, and Mathieu Hautefeuille