Main description:

The epigenetic regulation plays an important role in normal development and maintenance of tissue specific genes expression in humans and the disturbance of these patterns lead to changes involved in tumor formation. More recently, epigenetic changes have been observed in early stages of tumor development and together with the genetic alterations have been defined as abnormalities, necessary for cancer initiation and progression. In, Cancer Epigenetics: Methods and Protocols, expert researchers reviewed these epigenetics changes in different tumor types and described several technologies that are currently available to detect epigenetic changes. These technologies have lead to a better understanding of the processes in normal and cancerous cells. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Cancer Epigenetics: Methods and Protocols aids scientists in continuing to study epigenetic alterations used in clinical practice as biomarkers of early cancerous lesions or markers of progression and prognosis.

Contents:

Part I: Early Epigenetic Events in Cancer

1. Epigenetic Markers of Early Tumor Development

Ramona G. Dumitrescu

2. Epigenetics of Solid Cancer Stem Cells

Alok Mishra and Mukesh Verma

Part II: Cancer Specific Type Epigenetic Changes

3. DNA Methylation and Histone Modifications in Breast Cancer

Ramona G. Dumitrescu

4. DNA Methylation Changes in Prostate Cancer

Wolfgang Goering, Michael Kloth, Wolfgang A. Schulz

5. DNA Methylation in Promoter Region as Biomarkers in Prostate Cancer

Mihi Yang and Jong Y. Park

6. Epigenetics of Bladder Cancer

Wun-Jae Kim and Yong-June Kim

7. Epigenetics in Myeloid Malignancies

Stefan Deneberg

8. The Epigenetics of Brain Tumors

Adrian M. Dubuc, Stephen Mack, Alexander Unterberger, Paul A. Northcott and Michael D. Taylor

9. DNA Methylation Changes in Cervical Cancers

Qiang Lu, Dehua Ma, Shuping Zhao

10. Epigenetics of Colon Cancer

Sharad Khare and Mukesh Verma

11. Promoter Methylation in Head and Neck Tumorigenesis

Josena K. Stephen, Kang Mei Chen, Shaleta Havard, Glynis Harris, Maria J. Worsham

12. Epigenome and DNA Methylation in Oral Squamous Cell Carcinoma

Mario Perez-Sayans Garcia and Abel Garcia-Garcia

13. Clinical Implications of Epigenetic Alterations in Human Thoracic Malignancies: Epigenetic Alterations in Lung Cancer

Keiko Shinjo and Yutaka Kondo

14. The Role of microRNAs in the Management of Liver Cancer

Krishna K Banaudha and Mukesh Verma

15. Epigenetics in Ovarian Cancer

L.M.S. Seeber, P.J. van Diest

Part III: Methods and Technologies used for Epigenetic Changes Detection

16. DNA Methylation in Pancreatic Cancer: Protocols for the Isolation of DNA and Bisulfite Modification

Katja Biewusch, Marie Heyne, Robert Grutzmann, Christian Pilarsky

17.Detection and Analysis of DNA Methylation by Pyrosequencing

Hilary A. A. Colyer, Richard N. Armstrong, Dr. Daniel J. Sharpe and Ken I. Mills

18. Screening for miRNA Experssion Changes using Quantitative PCR (Q-PCR)

Richard N. Armstrong, Hilary A. A. Colyer, Ken. I. Mills

19. Genome-wide Methylation Analysis

Alexander Unterberger, Adrian M. Dubuc and Michael D. Taylor

20. Microarray for Epigenetic Changes: Gene Expression Arrays

Hilary A. A. Colyer, Richard N. Armstrong and Ken I. Mills

21. Detecting DNA Methylation using the Methylated CpG Island Amplification and Microarray Technique

Hiliary A. A. Colyer, Margaret Dellett and Ken I. Mills

Part IV. Factors that Influence the Epigenetic Changes in Cancer

22. Nutrigenomics-Implications for Breast and Colon Cancer Prevention

Gabriela Riscuta and Ramona G. Dumitrescu

23. Dietary and Lifestyle Factors of DNA Methylation

Unhee Lim and Min-Ae Song

24. Diet, Epigenetics and Cancer

Joseph L. Su

25. Role of Epigenetics in Cancer Health Disparities

Sulma I. Mohammed, Sanya Springfield, and Rina Das

26. Multifactorial Etiology of Gastric Cancer

Rosa A. Sierra, Jovanny Zabaleta

Part V. Epigenetic Epidemiology

27. Epigenetic Epidemiology for Cancer Risk: Harnessing Germline Epigenetic Variation

Kevin Brennan and James M. Flanagan

28. Epigenetic Biomarkers in Cancer Epidemiology

Mukesh Verma