Main description:

This new edition presents principle methods in capillary electrophoresis (CE) separation involving CZE, MEKC, MECC, NACE, and corresponding hyphenated techniques to organic mass spectrometry and ICP-MS. Recent developments in the techniques of single cell analysis, as well as derivation, enantioseparation or the use of ionic liquids, and the use of CZE for the separation of living cells are also highlighted. This book discusses various application methods for the analysis of small ions, organic acids, amino acids, and (poly)saccharides to peptides that are shown with pollutants and biomarkers in food and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Capillary Electrophoresis: Methods and Protocols, Second Edition covers a wide field of interests and will be especially great for beginners and students because of its combined focus on mini-reviews and application notes that will help them quickly get an overview of the field.

Contents:

Part I Principles and Instrumental

1. The CE-Way of Thinking: "All is Relative!"
Philippe Schmitt-Kopplin and Agnes Fekete

2. A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic

Behaviors of Small Molecules in Free Zone Electrophoresis

Philippe Schmitt-Kopplin and Agnes Fekete<

3. Derivatization in Capillary Electrophoresis

M. Luisa Marina and Maria Castro-Puyana

4. Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS

Peptide Analysis-Strategies and Implementation

Martin Pattky, Katalin Barkovits, Oliver Weiergraber, and Carolin Huhn

5. Micellar Electrokinetic Chromatography of Aminoglycosides

Ulrike Holzgrabe, Stefanie Schmitt, and Frank Wienen

6. Microemulsion Electrokinetic Chromatography

Wolfgang Buchberger

7. Non Aqueous Capillary Electrophoresis Mass Spectrometry

Christian W. Klampfl and Markus Himmelsbach

8. Ionic Liquids in Capillary Electrophoresis

Ulrike Holzgrabe and Joachim Wahl

9. CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve

Felix J. Kohl and Christian Neususs

10. Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry

Bernhard Michalke

11. Use of CE to Analyze Solutes in Pico- and Nano-Litre Samples from Plant Cells and

Rhizosphere

A. Deri Tomos

Part II Applications from Small to Macromolecules

12. Analysis of Small Ions with Capillary Electrophoresis

Jatinder Singh Aulakh, Ramandeep Kaur, and Ashok Kumar Malik

13. Metal Ions Analysis with Capillary Zone Electrophoresis

Ashok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur

14. Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis

Daniela Fico, Antonio Pennetta, and Giuseppe E. De Benedetto

15. Enantiomer Separations by Capillary Electrophoresis

Gerhard K. E. Scriba, Henrik Harnisch, and Qingfu Zhu

16. Capillary Electrophoresis of Mono- and Oligosaccharides

Mila Toppazzini, Anna Coslovi, Marco Rossi, Anna Flamigni, Edi Baiutti, and Cristiana Campa

17. Use of Capillary Electrophoresis for Polysaccharide Studies and Applications

Amelia Gamini, Anna Coslovi, Mila Toppazzini, Isabella Rustighi, Cristiana Campa, Amedeo Vetere,

and Sergio Paoletti

18. Separation of Peptides by Capillary Electrophoresis

Gerhard K. E. Scriba

19. Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae

sing Capillary Electrophoresis Approach

Pawel Pomastowski, Viorica Railean-Plugaru, and Boguslaw Buszewski

20. Capillary Electrophoretic Analysis of Classical Organic Pollutants

Ashok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur

21. Capillary Electrophoresis in Metabolomics

Tanja Verena Maier and Philippe Schmitt-Kopplin

22. Capillary Electrophoresis in Food and Foodomics

Clara Ibanez, Tanize Acunha, Alberto Valdes, Virginia Garcia-Canas, Alejandro Cifuentes, and

Carolina Simo

23. Capillary Electrophoresis in Wine Science

Christian Coelho, Franck Bagala, Regis Gougeon, and Philippe Schmitt-Kopplin