Main description:

This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.

Contents:

1. Carbohydrate-based Vaccines: An Overview

Julia Hutter and Bernd Lepenies

2. Glycans as Vaccine Antigens and Adjuvants: Immunological ConsiderationsStephanie Zimmermann and Bernd Lepenies

3. The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens

Li Xia and Jeffrey C. Gildersleeve

4. Antibody-carbohydrate Recognition from Docked Ensembles using

the AutoMap Procedure

Tamir Dingjan, Mark Agostino, Paul A. Ramsland, and Elizabeth Yuriev

5. Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens

Felix Broecker, Chakkumkal Anish, and Peter H. Seeberger

6. Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria

Guillaume Goyette-Desjardins, Rene Roy, and Mariela Segura

7. Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility

David Bitto, David J. Harvey, Steinar Halldorsson, Katie J. Doores, Laura K. Pritchard,

Juha T. Huiskonen,Thomas A. Bowden, and Max Crispin

8. N-Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIFRene Hennig , Erdmann Rapp, Robert Kottler, Samanta Cajic , Matthias Borowiak , and Udo Reichl

9. Temporary Conversion of Protein Amino Groups To Azides: A Synthetic Strategy For Glycoconjugate Vaccines Tomasz Lipinski and David R. Bundle

10. Gold Nanoparticles as

Carriers For Synthetic Glycoconjugate Vaccines

Fabrizio Chiodo and Marco Marradi

11. Identification and Characterization of Carbohydrate-Based Adjuvants

Timo Johannssen and Bernd Lepenies

12. Characterization of Carbohydrate Vaccines by NMR Spectroscopy

Francesco Berti and Neil Ravenscroft

13. Characterisation of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods Stephen E. Harding, Ali Saber Abdelhameed, Richard B. Gillis, Gordon A. Morris, and Gary G. Adams

14. Glycoconjugate Vaccines: The Regulatory Framework

Christopher Jones