Main description:

Embryonic stem cells and adult stem cells are the two major types of stem cells that have been used for experimental and clinical studies. Embryonic stem cells are totipotent cells that have the capability to differentiate into any type of cell in the body. In Cellular Cardiomyoplasty: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study cellular cardiomyoplasty. Methods and techniques described in this volume use only adult stem cells or adult progenitor cells.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and Practical, Cellular Cardiomyoplasty: Methods and Protocols will benefit the cardiologist, cardiothoracic surgeons, biologist (cell, molecular, or structural), biochemist, and physiologist who are interested in understanding and treating damaged myocardium and failing heart.

Contents:

1. Cellular Cardiomyoplasty: Its Past, Present and Future

Elizabeth K. Lamb, Grace W. Kao, and Race L. Kao

2. Skeletal Muscle Stem Cells

Grace W. Kao, Elizabeth K. Lamb, and Race L. Kao

3. Bone Marrow Stem Cells

Minh Ngoc Duong, Yu-Ting Ma, and Ray C.J. Chiu

4. Adipose Tissue Derived Mesenchymal Stem Cells: Isolation, Expansion and Characterization

Miriam Arana, Manuel Mazo, Pablo Aranda, Beatriz Pelacho and Felipe Prosper

5. Cardiac Side Population Cells and Sca-1-positive Cells

Toshio Nagai, Katsuhisa Matsuura, and Issei Komuro

6. Two Step Protocol for Isolation and Culture of Cardiospheres

Lijuan Chen, Yaohua Pan, Lan Zhang, Yingjie Wang, Neal Weintraub, and Yaoliang Tang

7. Generation of Human iPSCs From Human Peripheral Blood Mononuclear Cells using Non-Integrative Sendai Virus in Chemically Defined Conditions

Jared M. Churko, Paul W. Burridge, and Joseph C. Wu

8. Identification of Stem Cells after Transplantation

Yingjie Wang, Lan Zhang, Yaohua Pan, Lijuan Chen, Neal Weintraub, Yaoliang Tang

9. Methods to Study the Proliferation and Differentiation of Cardiac Side Population (CSP) Cells

Konstantina-Ioanna Sereti, Angelos Oikonomopoulos, Kazumasa Unno, and Ronglih Liao

10. Immune Responses After Mesenchymal Stem Cell Implantation

Rony Atoui and Ray C.J. Chiu

11. Route of Delivery, Cell Retention and Efficiency of Polymeric Microcapsules in Cellular Cardiomyoplasty

Alice Le Huu, Arghya Paul, Satya Prakash, and Dominique Shum-Tim

12. Angiogenic Nanodelivery Systems for Myocardial Therapy

Arghya Paul, Dominique Shum-Tim, and Satya Prakash

13. Bio-Hybrid Tissue Engineering for Cellular Cardiomyoplasty: Future Directions

Juan Carlos Chachques

14. Decellularized Whole Heart for Bioartificial Heart

Hug Aubin, Alexander Kranz, Joern Hulsmann, Artur Lichtenberg, and Payam Akhyari

15.Clinical Trials of Cardiac Repair with Adult Bone Marrow-Derived Cells

Vinodh Jeevanantham, Mohammad R. Afzal, Ewa K. Zuba-Surma, and Buddhadeb Dawn

16. Clinical Study Using Adipose Derived Mesenchymal-like Stem Cells in Acute Myocardial Infarction and Heart Failure

I.A. Panfilov, R. de Jong, S. Takashima, H.J. Duckers