Main description:

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.

Contents:

1. Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast
Ge Lin, Kristen Martins-Taylor, and Ren-He Xu

2. Isolation and Maintenance of Mouse Epiblast Stem Cells
Josh G. Chenoweth and Paul J. Tesar

3. Functional Assays for Hematopoietic Stem Cell Self-Renewal
John M. Perry and Linheng Li

4. Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells (MAPC) from Rat Bone Marrow
Kartik Subramanian, Martine Geraerts, Karen A. Pauwelyn, Yonsil Park, D. Jason Owens, Manja Muijtjens, Fernando Ulloa-Montoya, Yeuhua Jiang, Catherine M. Verfaillie, and Wei-Shou Hu

5. Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In vitro
Yan Shi

6. Mesoderm Cell Development from ES Cells
Takumi Era

7. Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells
Shi-Jiang Lu, Qiang Feng, Jennifer S. Park, and Robert Lanza

8. Directed Differentiation of Neural and Subtype Specific Neuronal Cells from hESCs
Bao-Yang Hu and Su-Chun Zhang

9. Directing Human Embryonic Stem Cells to a Retinal Fate
Deepak Lamba, Julianne Gust, and Thomas A. Reh

10. Bovine Somatic Cell Nuclear Transfer
Pablo J. Ross and Jose B. Cibelli

11. Cell Fusion Induced Reprogramming
Jeong Tae Do and Hans R. Schoeler

12. An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells
Prashant Mali, Zhaohui Ye, Bin-Kuan Chou, Jonathan Yen, and Linzhao Cheng

13. Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells
Caroline Desponts and Sheng Ding

14. Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression
Huafeng Xie, Catherine V. Laiosa, andThomas Graf

15. Reprogramming of B Cells
Cesar Cobaleda

16. Adult Cell Fate Reprogramming: Converting Liver to Pancreas
Irit Meivar-Levy and Sarah Ferber

17. In vitro Reprogramming of Pancreatic Cells to Hepatocytes
Daniel Eberhard, Kathy O'Neill, Zoe D. Burke, and David Tosh

18. Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches
Wenlin Li and Sheng Ding

19. Small Molecule Screen in Zebrafish and HSC Expansion
Eirini Trompouki and Leonard I. Zon

20. Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation
Kathleen M. Munson and Jing-Ruey J. Yeh