Main description:

This
volume covers multidisciplinary approaches on various aspects of Chikungunya
Virus (CHIKV) research that was brought together from leading laboratories
across the globe. Topics covered include techniques in clinical and diagnostic
virology, basic protocols in cell and virus culture, bioinformatics, and
proteomics approaches in cellular response studies. Chikungunya Virus:
Methods and Protocols also discusses methods in immunology and animal model
studies, as well as different strategies of antivirals and vaccine development
for therapeutics against CHIKV infection. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary material and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.

Thorough and practical, Chikungunya Virus: Methods and Protocols is a
useful and comprehensive resource for CHIKV researchers and anyone interested
in learning more about the Chikungunya Virus.

Contents:

Part I Clinical and Diagnostic Virology

1. Evolution and Epidemiology
of Chikungunya Virus
Giada Rossini, Maria Paola Landini, and
Vittorio Sambri

2. Detection
and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay
Seok Mui Wang, Ummul Haninah Ali, Shamala
Devi Sekaran, and Ravindran Thayan

3. Advanced Genetic Methodologies
in Tracking Evolution and Spread of Chikungunya Virus
Hapuarachchige Chanditha Hapuarachchi and
Kim-Sung Lee

4. Synthetic
Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with
and without Neurological Complications
Rajpal S. Kashyap, Shradha S. Bhullar,
Nitin H. Chandak, and Girdhar M. Taori

5. Expression and Purification of E2 Glycoprotein from Insect
Cells (Sf9) for Use in Serology
Chong Long Chua, I-Ching Sam, and Yoke
Fun Chan

6. Diagnostic Methods for CHIKV Based on Serological Tools
Paolo Gaibani, Maria Poala Landini, and
Vittorio Sambri

7. Utilization and Assessment of Throat Swab and Urine Specimens
for Diagnosis of Chikungunya Virus Infection
Chandrashekhar G. Raut, H. Hanumaiah, and
Wrunda C. Raut

Part II Cell Culture, Virus Replication,
and Cellular Responses

8. Propagation
of Chikungunya Virus Using Mosquito Cells
Swee Kim Ang, Shirley Lam, and Justin
Jang Hann Chu

9. Infectious
Viral Quantification of Chikungunya Virus - Virus Plaque Assay
Parveen Kaur, Regina Ching Hua Lee, and
Justin Jang Hann Chu

10. Molecular
Epidemiology of Chikungunya Virus by Sequencing
Ravindran Thayan, Mohd Apandi Yusof,
Zainah Saat, Shamala Devi Sekaran, and Seok Mui Wang

11. Chikungunya Virus Infection of Aedes Mosquitoes
Hui Vern Wong, Yoke Fun Chan, I-Ching
Sam, Wan Yusof Wan Sulaiman, and Indra Vythilingam

12. Analysis
of CHIKV in Mosquitoes Infected via Artificial Blood Meal
Jeremy P. Ledermann and Ann M. Powers

13. Chikungunya
Virus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by Immunofluorescence
Assay
Meng Ling Moi and Tomohiko Takasaki

14. Virus Isolation and Preparation of Sucrose-Banded
Chikungunya Virus Samples for Transmission Electron Microscopy
Chang-Kweng Lim

15. Viral-Host
Protein Interaction Studies Using Yeast Two-Hybrid Screening Method
Namrata Dudha and Sanjay Gupta

16. Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection:
Preparation of Peptides for Analysis
Atchara Paemanee, Nitwara Wikan, Sittiruk
Roytrakul, and Duncan R. Smith

17. Bioinformatics
Based Approaches to Study Virus-Host Interactions During Chikungunya Virus Infection
Sreejith Rajasekharan and Sanjay Gupta

18. T-Cell Epitope Prediction of
Chikungunya Virus
Christine Loan Ping Eng, Tin Wee Tan, and
Joo Chuan Tong

Part III Immunology and Animal Model
Studies

19. Mouse
Models of Chikungunya Virus
Lara J. Herrero, Penny A. Rudd, Xiang
Liu, Stefan Wolf, and Suresh Mahalingam

20. Generation
of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY
Hybridoma Cloning Kit
Chow Wenn Yew and Yee Joo Tan

21. Immunohistochemical
Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded
Tissues
Chun Wei Chiam, I-Ching Sam, Yoke Fun
Chan, Kum Thong Wong, and Kien Chai Ong

Part IV Antivirals and Vaccines

22. Antiviral Strategies against Chikungunya Virus
Rana Abdelnabi, Johan Neyts, and Leen
Delang

23. A Real Time Cell Analyzing Assay for Identification
of Novel Antiviral Compounds against Chikungunya Virus
Keivan Zandi

24. Using Bi-Cistronic Baculovirus Expression Vector System to Screen the Compounds
that Interfere the Infection of Chikungunya Virus
Szu-Cheng Kuo, Chao-Yi Teng, Yi-Jung Ho,
Ying-Ju Chen, and Tzong-Yuan Wu

25. Neutralization
Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test
Nor Azila Muhammad Azami, Meng Ling Moi,
and Tomohiko Takasaki

26. Reverse
Genetics Approaches for Chikungunya Virus
Patchara Phuektes and Justin Jang Hann
Chu

27. Production
of Chikungunya Virus-Like Particles and Subunit Vaccines in Insect Cells
Stefan W. Metz and Gorben P. Pijlman

28. Protocols for Developing Novel Chikungunya Virus DNA
Vaccines
Christopher Chung, Kenneth E. Ugen,
Niranjan Y. Sardesai, David B. Weiner, and Kar Muthumani