Main description:

This volume highlights the molecular and cellular methods used in studying Chronic Myeloid Leukimia (CML) pathogenesis and stem cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Chronic Myeloid Leukemia: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

1. Molecular Detection of BCR-ABL in Chronic Myeloid Leukemia

Ya-Zhen Qin and Xiao-Jun Huang

2. Induction of Chronic Myeloid Leukemia in Mice

Haojian Zhang and Shaoguang Li

3. CML Mouse Model Generated from Leukemia Stem Cells

Yiguo Hu

4. Immunological Analyses of Leukemia Stem Cells

Kazuhito Naka and Yoshihiro Takihara

5. Cell Cycle Analysis of CML Stem Cells using Hoechst33342 and Propidium Iodide

Ngoc DeSouza, Megan Zhou, and Yi Shan

6. Histological and In Vivo Microscopic Analysis of the Bone Marrow Microenvironment in a Murine Model of Chronic Myelogenous Leukemia

Eva S. Weissenberger and Daniela S. Krause

7. The Culture Repopulation Ability (CRA) Assay and Incubation in Low Oxygen to Test Anti-Leukemic Drugs on Imatinib-resistant CML Stem-like Cells

Giulia Cheloni and Michele Tanturli

8. Tumor Suppressor Analysis in CML

Oliver Herrmann and Mirle Schemionek

9. Detecting Autophagy and Autophagy Flux in Chronic Myeloid Leukemia Cells Using a Cyto ID Fluorescence Spectrophotometric Assay

Sujuan Guo, Kevin Pridham, and Zhi Sheng

10. DREAM, A Simple Method for DNA Methylation Profiling by High Throughput Sequencing

Jaroslav Jelinek and Jozef Madzo

11. ChIP-seq Analysis of Human Chronic Myeloid Leukemia Cells

Lars Anders and Zhaodong Li

12. Quantitative Proteomics Analysis of Leukemia Cells

Sebastian Halbach, Joern Dengjel, and Tilman Brummer

13. A Convenient Cell Culture Model for CML Acquired Resistance through BCR-ABL Mutations

Zhiqiang Wang and WenYong Chen

14. High Throughput Screening of Tyrosine Kinase Inhibitor Resistant Genes in CML

Leyuan Ma, Justine Roderick, Michelle A. Kelliher, and Michael R. Green

15. Biological Analysis of Human CML Stem Cells; Xenograft Model of Chronic Phase Human Chronic Myeloid Leukemia

Sheela A. Abraham

16. New Mouse Models to Investigate the Efficacy of Drug Combinations in Human Chronic Myeloid Leukemia

Hanyang Lin, Adrian Woolfson, and Xiaoyan Jiang

17. miRNome Analysis of CML Cells

Yadong Yang, Nan Ding, Xunong Dong, Xiangdong Fang

18. An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells

Farah Nassar, Rabab El Eit, and Rihab Nasr

19. Single Cell Cytokine Profiling to Investigate Cellular Functional Diversity in Hematopoietic Malignancies

Jonathan J. Chen, Minsuk Kwak, and Rong Fan