Main description:

This book presents a series of protocols in multiple disease areas affected by the aging process along with several methods which have shown progress in nutrient- or intervention-based approaches to maximize the healthspan. Of interest to researchers in the areas of chronic disease, gerontology, physical exercise, and nutrition as well as to clinical scientists, physicians, and pharmacologists, this volume also provides important information on disease mechanisms and novel drug targets as each protocol will be presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Clinical and Preclinical Models for Maximizing Healthspan: Methods and Protocols is an ideal guide for preclinical and clinical researchers working toward the discoveries needed to lead to a world society in which individuals are not only living longer lives but more productive and healthier ones.

Contents:

Part I: Reviews

1. The Impact of New Biomarkers and Drug Targets on Age-Related Disorders

Paul C. Guest

2. Vitamin D and Muscle Sarcopenia in Aging

Behnaz Abiri and Mohammadreza Vafa

3. Quantitative Assays of Plasma Apolipoproteins

Anne Poljak, Mark W. Duncan, Tharusha Jayasena, and Perminder S. Sachdev

4. Protocol for the Use of the Ketogenic Diet in Preclinical and Clinical Practice

Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai

Part II: Protocols

5. An In Vivo/Ex Vivo Study Design to Investigate Effects of Chronic Conditions and Therapeutic Compounds on Adipose Stem Cells in Animal Models

Hanel Sadie-Van Gijsen, Liske Kotze-Hoerstmann, and Barbara Huisamen

6. Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations

Yashar Mehrbani Azar, Maria Jacoba Kruger, Dalene de Swardt, Michelle Maartens, Ascentia Mathapelo Seboko, William Frank Ferris, and Mari van de Vyver

7. Investigating Alcohol Behavior and Physiology Using Drosophila Melanogaster

Aliza K. De Nobrega, Kristine V. Luz, Katherine N. Lyons, and Lisa C. Lyons

8. Using Genome-Editing Tools to Develop a Novel In Situ Coincidence Reporter Assay for Screening ATAD3A Transcript Inhibitors

Liwei Lang and Yong Teng

9. Visualizing and Evaluating Cancer Cell Growth and Invasion by a Novel 3D Culture System

Chloe Shay, Liwei Lang, and Yong Teng

10. Exploiting Plug-and-Play Electrochemical Biosensors to Determine the Role of FGF19 in Sorafenib-Mediated Superoxide and Nitric Oxide Production in Hepatocellular Carcinoma Cells

Lixia Gao and Yong Teng

11. Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines

Chareeporn Akekawatchai, Sittiruk Roytrakul, Narumon Phaonakrop, Janthima Jaresitthikunchai, and Sarawut Jitrapakdee

12. Real-Time PCR Analysis of Metabolism-Related Genes in a Long-Lived Model of C. Elegans

Sumino Yanase

13. Clinical Assessment of the NADome as Biomarkers for Healthy Aging

Tharusha Jayasena, Sonia Bustamante, James Clement, Robert Welschinger, Gideon A. Chaplan, Perminder S. Sachdev, and Nady Braidy

14. 2D Gel Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Eye Lens to Identify Biomarkers of Age-Related Cataract

Paul C. Guest

15. Testing the Effects of Dietary Seafood Consumption on Depressive Symptoms

Maximus Berger, G. Paul Amminger, Robyn McDermott, Paul C. Guest, and Zoltan Sarnyai

16. Assays for Monitoring the Effects of Nicotinamide Supplementation on Mitochondrial Activity in Saccharomyces Cerevisiae

Ivan Orlandi and Marina Vai

17. Measurement of a Surrogate Biomarker for Arginine Vasopressin Secretion is Association with Physiometric and Molecular Biomarkers of Aging

Paul C. Guest, Hans-Gert Bernstein, Henrik Dobrowolny, Katrin Borucki, Sabine Westphal, and Johann Steiner

18. Liquid Chromatography Tandem Mass Spectrometry Analysis of Proteins Associated with Age-Related Disorders in Human Pituitary Tissue

Paul C. Guest and Daniel Martins de-Souza

19. Coenzyme Q10 Assessment and the Establishment of a Neuronal Cell Model of CoQ10 Deficiency

Robert Heaton, Kate Duberley, and Iain P. Hargreaves

20. Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer

Justin Darcy, Chih-Hao Wang, and Yu-Hua Tseng

21. Quantitative Analysis of DNA Methylation by Bisulfite Sequencing

Vasily V. Ashapkin, Lyudmila I. Kutueva, and Boris F. Vanyushin

22. Histomorphometric Analysis of Anti-Aging Properties on Rat Skin

Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, and Eka Pramyrtha Hestianah

23. Integration of qRT-PCR and Immunohistochemical Techniques for mRNA Expression and Localization of m1AChR in the Brain of Aging Rat

S. Asha Devi and S. Abhijit

24. Randomized Study Design to Test Effects of Vitamin D and Omega-3 Fatty Acid Supplementation as Adjuvant Therapy in Colorectal Cancer Patients

Fatemeh Haidari, Behnaz Abiri, Masood Iravani, Kambiz Ahmadi-Angali, and Mohammadreza Vafa

25. Effect of Vitamin D Supplementation on Muscle Strength, Muscle Function, and Body Composition in Vitamin D-Deficient Middle-Aged Women

Behnaz Abiri, Mohsen Dehghani, and Mohammadreza Vafa

26. The Association of Food Intake and Physical Activity with Body Composition, Muscle Strength, and Muscle Function in Postmenopausal Women

Mohammadreza Vafa, Behnaz Abiri, and Mohsen Dehghani

27. Absolute Quantification of Plasma Apolipoproteins for Cardiovascular Disease Risk Prediction

Betul Ozdemir, Zeliha Selamoglu, and Nady Braidy

28. Multiplex Analysis of Circulating Hormone Levels in Rat Models of Age-Related Diseases

Paul C. Guest

29. Proteomic Analysis of Brain Tissue from a Chronic Model of Stress Using a Combined 2D Gel Electrophoresis and Mass Spectrometry Approach

Paul C. Guest

30. Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging

Paul C. Guest, Hassan Rahmoune, and Daniel Martins-de Souza

31. Brain Proteomic Analysis on the Effects of the Antidepressant Fluoxetine

Paul C. Guest

32. MK-801 Treatment of Oligodendrocytes as a Cellular Model of Aging

Paul C. Guest