Main description:

This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.

Contents:

1. A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care

Alain R. Thierry

2. COLD-PCR: Applications and Advantages

Zhuang Zuo and Kausar J. Jabbar

3. PCR-Based Detection of DNA Copy Number Variation

Meenakshi Mehrotra

4. Emulsion PCR: Techniques and Applications

Rashmi Kanagal-Shamanna

5. Digital PCR: Principles and Applications

Rashmi Kanagal-Shamanna

6. Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics

Sanam Loghavi

7. High Resolution Melt Curve Analysis in Cancer Mutation Screen

Meenakshi Mehrotra and Keyur P. Patel

8. Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations

Khedoudja Nafa, Meera Hameed, and Marie E. Arcila

9. Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis

Alberto Paniz Mondolfi and Rajesh R. Singh

10. Microfluidics-Based PCR for Fusion Transcript Detection

Hui Chen

11. Polymerase Chain Reaction Diagno

sis of Leishmaniasis: A Species-Specific Approach

&n

bsp; Eglys Gonzalez-Marcano, Hirotomo Kato, Juan Luis Concepcion, Maria Elizabeth Marquez, and Alberto Paniz-Mondolfi

12. Detection of Trypanosoma cruzi by Polymerase Chain Reaction

Maria Elizabeth Marquez, Juan Luis Concepcion, Eglys Gonzalez-Marcano, and Alberto Paniz-Mondolfi

13. PCR Techniques in Next-Generation Sequencing

Rashmi S. Goswami

14. Single Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics

Chi Young Ok, Rajesh R. Singh, and Alaa A. Salim

15. Quantitative Real Time PCR: Recent Advances

Charanjeet Singh and Sinchita Roy-Chowdhuri

16. PCR Techniques in Char

acterizing DNA Methylation

Khalida Wani and Kenneth D. Aldape