Main description:

This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.

Contents:

1. Methods of Development of Biparental Mapping Populations in Horticultural Crops

Pasquale Tripodi

2. Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits

Isidore Diouf and Laura Pascual

3. Using Metabolomics to Assist Plant Breeding

Saleh Alseekh and Alisdair R. Fernie

4. High Throughput DNA Isolation in Vegetable Crops for Genomics Applications

Pasquale Tripodi and Giovanna Festa

5. High Resolution Melting Analysis as a Tool for Plant Species Authentication Liliana Grazina, Joana Costa, Joana S. Amaral, and Isabel Mafra

6. Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping Methods

Zhangsheng Zhu, Binmei Sun, and Jianjun Lei

7. Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome

Fernando J. Yuste-Lisbona, Jose M. Jime nez-Go mez, Carmen Capel, and Rafael Lozano

8. Association Mapping in Plants

Pawan L. Kulwal and Ravinder Singh

9. Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs)

Contaldi Felice, Cappetta Elisa, and Esposito Salvatore

10. Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis Teresa Rosa Galise, Salvatore Esposito, and Nunzio D'Agostino

11. RNA Interference (RNAi) in Tomato Crop Reseserch

Pasquale Termolino

12. Protoplast Based Method for Genome Editing in Tetraploid Potato

Alessandro Nicolia, Ann-Sofie Falt, Per Hofvander, and Mariette Andersson

13. The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronaria

Andrea Copetta and Marina Laura

14. Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant

Edgar Garcia-Fortea, Ana Garcia-Perez, Esther Gimeno-Paez, Marina Martinez-Lopez, Santiago Vilanova, Pietro Gramazio, Jaime Prohens and Mariola Plazas

15. Assembly of TALEN and mTALE-Act for Plant Genome Engineering

Aimee A. Malzahn and Yiping Qi

16. Genome Editing to Achieve the Crop Ideotype in Tomato

Tomas Cermak, Karla Gasparini, Zoltan Kevei, and Agustin Zsoegoen

17. Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging

Gernot Bodner, Mouhannad Alsalem, and Alireza Nakhforoosh

18. Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis

Federico Pallottino, Simone Figorilli, Cristina Cecchini, and Corrado Costa