Main description:

This book reviews diversity-oriented synthesis (DOS), a leading and dynamic area in organic chemistry and drug discovery. It first covers key chemical methods for the generation of small molecules according to DOS principles as well as important classes of molecules generated through DOS approaches. The concept of diversity-oriented synthesis is then expanded to the description of chemical libraries. The book also examines modern approaches for screening DOS libraries and concludes with significant applications of DOS libraries and small molecules in the fields of drug discovery and chemical biology.

Contents:

CONTRIBUTORS xv FOREWORD xix PREFACE xxi ABBREVIATIONS xxv 1 The Basics of Diversity-Oriented Synthesis 1 Kieron M. G. O'Connell, Warren R. J. D. Galloway, and David R. Spring 1.1 Introduction, 1 1.2 What Is Diversity-Oriented Synthesis?, 1 1.3 Small Molecules and Biology, 2 1.4 Comparing DOS, TOS, and Combinatorial Chemistry: Focused Library Synthesis, 4 1.5 Molecular Diversity, 5 1.6 Molecular Diversity and Chemical Space, 8 1.7 Synthetic Strategies for Creating Molecular Diversity, 8 1.8 Reagent-Based Approaches to Diversity Generation, 11 1.9 Substrate-Based Approach to Skeletal Diversity Generation, 19 1.10 Other Build/Couple/Pair Examples, 19 1.11 Concluding Remarks, 24 PART I CHEMICAL METHODOLOGY IN DIVERSITY-ORIENTED SYNTHESIS 2 Strategic Applications of Multicomponent Reactions in Diversity-Oriented Synthesis 29 John M. Knapp, Mark J. Kurth, Jared T. Shaw, and Ashkaan Younai 2.1 Introduction, 29 2.2 MCR Products for HTS, 31 2.3 MCRs as Starting Points for DOS, 39 2.4 Conclusions, 55 3 Cycloaddition Reactions in Diversity-Oriented Synthesis 59 Giovanni Muncipinto 3.1 Introduction, 59 3.2 [4+2] Cycloaddition Reactions, 60 3.3 1,3-Dipolar Cycloaddition Reactions, 70 3.4 Miscellaneous Cycloadditions, 83 3.5 Conclusions, 91 4 Phosphine Organocatalysis as a Platform for Diversity-Oriented Synthesis 97 Zhiming Wang and Ohyun Kwon 4.1 Introduction, 97 4.2 DOS Using Phosphine Organocatalysis, 100 4.3 Skeletal Diversity Based on a Phosphine Catalysis/Combinatorial Scaffolding Strategy, 116 4.4 A DOS Library Based on Phosphine Organocatalysis: Biological Screening, Analog Synthesis, and Structure Activity Relationship Analysis, 121 4.5 Conclusions, 129 5 Domino Reactions in Library Synthesis 135 Matthew G. LaPorte, John R. Goodell, Sammi Tsegay, and Peter Wipf 5.1 Introduction, 135 5.2 Pericyclic Domino Reactions, 136 5.3 Anionic Domino Reactions, 150 5.4 Transition-Metal-Mediated Domino Reactions, 159 5.5 Radical Domino Reactions, 165 5.6 Conclusions, 174 6 Diversity-Oriented Synthesis of Amino Acid Derived Scaffolds and Peptidomimetics: A Perspective 177 Andrea Trabocchi 6.1 Introduction, 177 6.2 Definition and Classification of Peptidomimetics, 179 6.3 Early Combinatorial Approaches to Peptidomimetic Scaffolds, 180 6.4 Amino Acid Derived Scaffolds, 183 6.5 Macrocyclic Peptidomimetic Scaffolds, 194 6.6 Conclusions, 197 7 Solid-Phase Synthesis Enabling Chemical Diversity 201 Nadezda Canka!rova and Viktor Krch!nak 7.1 Introduction, 201 7.2 Skeletal Diversity, 203 7.3 Stereochemical Diversity, 234 7.4 Appendage Diversity, 238 7.5 Build/Couple/Pair Strategy, 239 7.6 Scaffold Hopping, 243 7.7 Conclusions, 249 8 Macrocycles as Templates for Diversity Generation in Drug Discovery 253 Eric Marsault 8.1 Introduction, 253 8.2 Challenges Associated with Macrocycles, 254 8.3 Macrocyclic Peptides, 259 8.4 Peptidomimetic Macrocycles, 265 8.5 Diversity-Oriented Strategies Based on Nonpeptidic Natural Product Scaffolds, 273 8.6 Conclusions, 281 PART II CHEMICAL LIBRARIES AND DIVERSITY-ORIENTED SYNTHESIS 9 Diversity-Oriented Synthesis of Natural Product Like Libraries 291 Mark Dow, Francesco Marchetti, and Adam Nelson 9.1 Introduction, 291 9.2 Libraries Inspired by Natural Product Scaffolds, 292 9.3 Folding Pathways in the Synthesis of Natural Product Like Libraries, 297 9.4 Branching Pathways in the Synthesis of Natural Product Like Libraries, 305 9.5 Oligomer-Based Approaches to Natural Product Like Libraries, 312 9.6 Summary, 320 10 Chemoinformatic Characterization of the Chemical Space and Molecular Diversity of Compound Libraries 325 Jose Luis Medina-Franco 10.1 Introduction, 325 10.2 Concept of Chemical Space, 326 10.3 General Aspects of Chemoinformatic Methods to Analyze the Chemical Space, 327 10.4 Chemoinformatic-Based Analysis of Libraries using Different Representations, 328 10.5 Recent Trends in Computational Approaches to Characterize Compound Libraries, 344 10.6 Concluding Remarks, 345 11 DNA-Encoded Chemical Libraries 353 Luca Mannocci 11.1 Introduction, 353 11.2 DNA-Encoded Chemical Libraries, 357 11.3 Selection and Decoding, 386 11.4 Drug Discovery by DNA-Encoded Chemical Libraries, 388 11.5 DNA-Encoded Chemical Libraries: Prospects and Outlook, 391 11.6 Conclusions, 393 PART III SCREENING METHODS AND LEAD IDENTIFICATION 12 Experimental Approaches to Rapid Identification, Profiling, and Characterization of Specific Biological Effects of DOS Compounds 403 Eduard A. Sergienko and Susanne Heynen-Genel 12.1 Introduction, 403 12.2 Basic Principles of HTS, 405 12.3 Common Assay Methods and Techniques, 415 12.4 Future Perspectives, 428 13 Small-Molecule Microarrays 431 Hongyan Sun 13.1 Introduction, 431 13.2 Chemical Library Design and Synthesis, 432 13.3 Fabrication of SMMs, 438 13.4 Applications of SMM, 446 13.5 Summary and Outlook, 451 14 Yeast as a Model in High-Throughput Screening of Small-Molecule Libraries 455 Irene Stefanini, Carlotta De Filippo, and Duccio Cavalieri 14.1 Introduction, 455 14.2 Chemical Genetics and S. cerevisiae, 461 14.3 Chemical Genomics and S. cerevisiae, 471 14.4 Conclusions: The Route of Drug Discovery with the Budding Yeast, 477 15 Virtual Screening Methods 483 Jurgen Bajorath 15.1 Introduction, 483 15.2 Basic Virtual Screening Concepts, 484 15.3 Molecular Similarity in Virtual Screening, 487 15.4 Spectrum of Virtual Screening Approaches, 489 15.5 Docking, 490 15.6 Similarity Searching, 491 15.7 Compound Classification, 496 15.8 Machine Learning, 498 15.9 Conclusions, 501 16 Structure Activity Relationship Data Analysis: Activity Landscapes and Activity Cliffs 507 Jurgen Bajorath 16.1 Introduction, 507 16.2 Numerical SAR Analysis Functions, 508 16.3 Principles and Intrinsic Limitations of Activity Landscape Design, 511 16.4 Activity Landscape Representations, 513 16.5 Defining and Identifying Activity Cliffs, 520 16.6 Activity Cliff Survey, 525 16.7 Activity Cliffs and SAR Information, 526 16.8 Concluding Remarks, 528 PART IV APPLICATIONS IN CHEMICAL BIOLOGY AND DRUG DISCOVERY 17 Diversity-Oriented Synthesis and Drug Development: Facilitating the Discovery of Novel Probes and Therapeutics 535 Jeremy R. Duvall, Eamon Comer, and Sivaraman Dandapani 17.1 Introduction, 535 17.2 Case Study 1: Inhibition of Cytokine-Induced -cell Apoptosis, 540 17.3 Case Study 2: Identification of Antimalarials, 548 17.4 Case Study 3: Targeting Protein Protein and Protein DNA Interactions, 558 17.5 Conclusions, 570 18 DOS-Derived Small-Molecule Probes in Chemical Biology 575 Nicholas Hill, Lingyan Du, and Qiu Wang 18.1 Introduction, 575 18.2 DOS-Derived Small-Molecule Probes, 576 18.3 Developing Small-Molecule Probes of Complex Biological Pathways, 576 18.4 Expanding the Collection of Important Biological Probes, 595 18.5 Developing Probes for Therapeutically Desirable Phenotypes, 603 18.6 Natural Product Inspired Small-Molecule Probes Developed from DOS and Biology-Oriented Synthesis, 606 18.7 Summary and Outlook, 611 References, 611 INDEX 619