Main description:

In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.

Contents:

1. DNA Electrophoresis: Historical and Theoretical Perspectives

Gareth A. Roberts and David T.F. Dryden

2. Basic DNA Electrophoresis in Molecular Cloning: a Comprehensive Guide for Beginners

Svetlana Makovets

3. Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-dimensional (2D) Native-native Agarose Gel Electrophoresis

Nicholas P. Robinson

4. Analysis of DNA Structures from Eukaryotic cells by Two-dimensional Native-native DNA Agarose Gel Electrophoresis

Andreas S. Ivessa

5. Directionality of Replication Fork Movement Determined by Two-dimensional Native-native DNA Agarose Gel Electrophoresis

Andreas S. Ivessa

6. Native/Denaturing Two-Dimensional DNA Electrophoresis And Its Application To The Analysis Of Recombination Intermediates

Jessica Lao, Shangming Tang and Neil Hunter

7. Plasmid DNA Topology Assayed by Two-dimensional Agarose Gel Electrophoresis

Jorge B. Schvartzman, Maria-Luisa Martinez-Robles, Pablo Hernandez, and Dora B. Krimer

8. A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-Quantitation of DNA Single-Strand Breaks

Brian Pachkowski and Jun Nakamura

9. Denaturing Gradient Gel Electrophoresis (DGGE)

Fiona Strathdee and Andrew Free

10. Polyacrylamide Temperature Gradient Gel Electrophoresis

Viktor Viglasky

11. Separation of DNA Oligonucleotides using Denaturing Urea-PAGE

Fiona Flett and Heidrun Interthal

12. Pulsed-field Gel Electrophoresis of Bacterial Chromosomes

Julia S. P. Mawer and David R. F. Leach

13. Resolution of Budding Yeast Chromosomes using Pulse Field Gel Electrophoresis

Aziz El Hage and Jonathan Houseley

14. Analysis of DNA Damage Via Single Cell Electrophoresis

Diana Anderson and Julian Laubenthal

15. Fluorescence in situ Hybridisation on Electrophoresed Cells to Detect Sequence Specific DNA Damage

Julian Laubenthal and Diana Anderson

16. Analysis of Protein-DNA Interactions using PAGE: Band-shift Assays

Lynn Powell

17. Assaying Cooperativity of Protein-DNA Interactions using Agarose Gel Electrophoresis

Tanya L. Williams and Daniel L. Levy

18. DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool

Fenfei Leng

19. Using PCR coupled to PAGE for Detection and Semi-quantitative Evaluation of Telomerase Activity

Laura Gardano