Main description:

This volume describes the methods of both in vivo and in vitro electroporation using ferrets, rats, mice, chickens, and zebrafish. Recent advances of experiments using the tetracycline-regulated gene expression and Tol2 transposon systems are also included. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Practical and authoritative, Electroporation Methods in Neuroscience serves to aid scientists in the further study into this crucially important way to study cells.

Contents:

1. In utero Electroporation of the Mouse Embryo

Tetsuichiro Saito

2. Exo utero Electroporation of the Mouse Embryo

Tetsuichiro Saito

3. In Vivo Electroporation of the Postnatal Rodent Forebrain

Camille Boutin and Harold Cremer

4. Electroporation in the Rodent Retina in vivo and in vitro

Takahiko Matsuda

5. Electroporation in the Developing Mouse Olfactory Bulb

Fumiaki Imamura and Charles A. Greer

6. Cell Migration Analysis of Cortical Interneurons after Electroporation

Fujio Murakami

7. Characterization of Newborn Interneurons In The Mouse Olfactory Bulb Using Postnatal Electroporation

Hiroo Takahashi, Sei-ichi Yoshihara, Ryo Asahina, Yoshinori Tamada, and Akio Tsuboi

8. Genetic Manipulation Of Gyrencephalic Carnivores

using in utero electroporation

Hiroshi Kawasaki

9. Electroporation for the Chick Embryonic CNS

Jun Hatakeyama and Kenji Shimamura

10. Targeting the Zebrafish Nervous System using In Vivo Electroporation

John H. Horne

11. Gene Transfer into Cultured Mammalian Embryos by Electroporation

Masanori Takahashi,Takako Kikkawa, and Noriko Osumi

12. Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Cultures

Naoyuki Matsumoto, Kensuke Sasaki, and Nobuhiko Yamamoto

13. Electroporation of Dissociated Hippocampal Neurons

Atsushi Baba and Tetsuichiro Saito

14. Tol2-Mediated Transgenesis And Its Application To Electroporation

Koichi Kawakami

15. Control of Gene Expression In Neurons by the use of in vivo Electroporation and the Tetracycline System

Tatsuya Sato, Yuko Muroyama, and Tetsuichiro Saito