Main description:

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

Contents:

Part I. Basic Concepts and Enhancement of DNA Transfer via Electroporation

1. Electroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology Developments

Lluis M. Mir

2. Electropermeabilization of the Cell Membrane

Justin Teissie

3. The Impact of Non-Electrical Factors on Electrical Gene Transfer

Jiemiao Hu, Jeffry Cutrera, and Shulin Li

4. Electroporation Formulation for Cell Therapy

Jiemiao Hu and Shulin Li

5. Short-Fragment DNA-Mediated in Vivo DNA Electroporation Delivery

Jinliang Peng, Yonggang Zhao, and Yuhong Xu

6. Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell Transfection

Shuyan Huang, Yingbo Zu, and Shengnian Wang

Part II. SiRNA Electrotransfer

7. SiRNA Delivery via Electropulsation: A Review of the Basic Processes

Muriel Golzio and Justin Teissie

8. Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips

Zewen Wei and Zhihong Li

9. Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells

Isabel Popp, Valentin Schatz, Alexander T. Prechtel, Alexander Steinkasserer, Christian Bogdan, and Jonathan Jantsch

10. Direct Imaging of siRNA Electrotransfer at the Single Cell Level

Justin Teissie and Muriel Golzio

11. Electroporation-Mediated siRNA Delivery into Tumors

Yoshifumi Takei

12. Substrate-Mediated, High Efficiency siRNA Electroporation

Hiroyuki Fujimoto and Hiroo Iwata

13. Intradermal Electroporation of RNA

Maria L. Knudsen, Karl Ljungberg, Peter Liljestroem, and Daniel X. Johansson

Part III. Electroporation-Mediated Delivery to Poorly Accessible and Fragile Tissues

14. Delivery of DNA into the Central Nervous System via Electroporation

Jochen De Vry, Tim Vanmierlo, Pilar Martinez-Martinez, Mario Losen, Yasin Temel, Harry Steinbusch, Marc De Baets, and Jos Prickaerts

15. DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes

John M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal, and Jeffrey H. Boatright

16. Delivery of Plasmid DNA into Developing Rat Teeth by Electroporation

Shaomian Yao, Michael L. Beckley, and Dawen Liu

17. Electroporation-Mediated Gene Delivery to the Lungs

Jennifer L. Young, Michael S. Barravecchia, and David A. Dean

18. Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion

David Machado-Aranda and Krishnan Raghavendran

19. In Vivo Electroporation-Mediated Gene Delivery to the Beating Heart

Hendrik T. Tevaearai, Amiq Gazdhar, M. N. Giraud, and Martin Fluck

Part IV. Treatment of Cancer in Large Animals and Humans via Electroporation Therapy

20. Managing Local Swelling Following Intratumoral Electro-Chemo-Gene Therapy

Jeffry Cutrera, Glenn King, Pamela Jones, Elias Gumpel, Xueqing Xia, and Shulin Li

21. Gene Electrotransfer in Clinical Trials

Julie Gehl

22. Electrochemotherapy in Veterinary Oncology: From Rescue to First-line Therapy

Enrico P. Spugnini and Alfonso Baldi

23. Low Energy DC Current Ablation in a Mouse Tumor Model

Prejesh Philips and Robert C. G. Martin

Part V. Applications of Electroporation for DNA Vaccination

24. Evolution of Electroporated DNA Vaccines

Andrea Keane-Myers and Matthew Bell

25. Clinical Development of Intramuscular Electroporation: Providing a "Boost" for DNA Vaccines

Amir S. Khan, Kate E. Broderick, and Niranjan Y. Sardesai

26. Electroporation-Mediated Administration of Candidate DNA Vaccines against HIV-1

Sandhya Vasan

27. Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates

Lucille Adam, Roger Le Grand, and Frederic Martinon

28. Intramuscular DNA Vaccination Protocols Mediated by Electric Fields Pieranna

Pieranna Chiarella and Emanuela Signori

29. DNA Electroporation of Multi-Agent Vaccines Conferring Protection Against Select Agent Challenge-TriGrid Delivery System

Andrea Keane-Myers, Matt Bell, Drew Hannaman, and Mark Albrecht

30. Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies

Pirouz Daftarian, Aram Vosoughi, and Vance Lemmon