Main description:

This detailed book provides an accessible compendium of up-to-date methods in the fields of environmental toxicology, molecular toxicology, and toxicogenomics. Organized into four major sections, the volume examines methods utilizing model animal species, such as nematode, fruit fly, mice, chicken, and amphibians, methods using plants to study chemical toxicity, applying the Ames assay to chemical mutagenicity study, as well as methods for environmental chemical analysis. Although this book is divided into these parts, the methods can be used across species. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Environmental Toxicology and Toxicogenomics: Principles, Methods, and Applications serves as a valuable resource for the scientific community, particularly for young scientists and graduate and undergraduate students, inspiring more research in the vitally important field of environmental toxicity, molecular toxicology, and toxicogenomics.

Contents:

Part I: Animal Toxicity

1. Detection of Caenorhabditis elegans Germ Cell Apoptosis Following Exposure to Environmental Contaminant Mixtures: A Crude Oil-Dispersant Mixture Example

Joseph Ryan Polli, Mary Farwell, and Xiaoping Pan

2. High-Throughput Measurement for Toxic Effects of Metal Mixtures in Caenorhabditis elegans

Kathy S. Xue and Lili Tang

3. Evaluations of Environmental Pollutants-Induced Mitochondrial Toxicity Using Caenorhabditis elegans as a Model System

Fuli Zheng, Michael Aschner, and Huangyuan Li

4. Methods to Assay the Behavior of Drosophila melanogaster for Toxicity Study

Guiran Xiao

5. Investigating the Joint Effects of Pesticides and Ultraviolet-B Radiation in Xenopus laevis and Other Amphibians

Shuangying Yu and Mike Wages

6. Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis

Xiaoping Pan and Baohong Zhang

7. Semi-Quantitative RT-PCR: An Effective Method to Explore the Regulation of Gene Transcription Level Affected by Environmental Pollutants

Fan Wang

8. Employing Multiple New Neurobiological Methods to Investigate Environmental Neurotoxicology in Mice

Ping Cai and Huangyuan Li

9. Analyses of Epigenetic Modification in Environmental Pollutants-Induced Neurotoxicity

Guangxia Yu, Zhenkun Guo, and Huangyuan Li

10. The Application of Omics Technologies in the Research of Neurotoxicology

Wenya Shao and Huangyuan Li

11. Flow Cytofluorometric Analysis of Molecular Mechanisms of Premature Red Blood Cell Death

Mohammad A. Alfhili and Myon Hee Lee

12. Practical Methods and Technologies in Environmental Epidemiology

Chuancheng Wu, Donghong Wei, Huangyuan Li, and Siying Wu

13. In Ovo Early-in-Life Inhalation Exposure to Gas/Aerosol with a Chicken Embryo Model

Qixiao Jiang, Xiaohui Xu, Hao Ni, Yajie Guo, Junhua Yuan, and Yuxin Zheng

14. DNA Damage in Liver Cells of the Tilapia Fish Oreochromis mossambicus Larva Induced by the Insecticide Cyantraniliprole at Sublethal Doses During Chronic Exposure

Yongmei Fan, Chengbin Xu, and Weiguo Miao

Part II: Plant Toxicity

15. Impact of Nanoparticles on Plant Growth, Development, and Biomass

Ilya N. Boykov and Baohong Zhang

16. Biochemical and Physiological Toxicity of Nanoparticles in Plant

Zhiyong Zhang and Baohong Zhang

17. Determination of Oxidative Stress and Antioxidant Enzyme Activity for Physiological Phenotyping During Heavy Metal Exposure

Samrana Zahir, Fan Zhang, Jinhong Chen, and Shuijin Zhu

18. Comprehensive Phytotoxicity Assessment Protocol for Engineered Nanomaterials

Lok R. Pokhrel, Chukwudi S. Ubah, and Sina Fallah

19. Detection of Cadmium Toxicity in Plant

Xiaoxiao Liu, Lina Yin, Shiwen Wang, and Zhiyong Zhang

Part III: Microbe Toxicity

20. Mutagenicity Evaluation of Nanoparticles by the Ames Assay

Xiaoping Pan

Part IV: Extraction and Detection Analytical Methods

21. Dispersive Solid Phase Extraction of Multiresidue Pesticides in Food and Water Samples

Hongxia Guan

22. Quantitative Analysis of Multiresidue Pesticides Using Gas Chromatography/Mass Spectrometry

Qingsong Cai and Hongxia Guan

23. Determination of the N-Nitroso Compounds in Mouse Following RDX Exposure

Xiaoping Pan

24. Determination of Metal Content in Drosophila melanogaster During Metal Exposure

Guiran Xiao

25. Microplastics: A Review of Methodology for Sampling and Characterizing Environmental and Biological Samples

Christiana H. Shoopman and Xiaoping Pan