Main description:

Reflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Dependable and easy to follow, Epidermal Cells: Methods and Protocols, Third Edition serves researchers working to accelerate the work in this vital field of study.

Contents:

1. Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage

Igor Kogut, Dennis R. Roop, and Ganna Bilousova

2. Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells

Fahad K. Kidwai, Tong Cao, and Kai Lu

3. Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds

Dongxia Ye and Antonio Peramo

4. Growth and Differentiation of HaCaT Keratinocytes

Van G. Wilson

5. Transgene Delivery to Cultured Keratinocytes Via Replication-Deficient Adenovirus Vectors

Vincent P. Ramirez and Brian J. Aneskievich

6. Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq

Jason M. Rizzo and Satrajit Sinha

7. Analysis and Meta-Analysis of Transcriptional Profiling in Human Epidermis

Claudia Mimoso, Ding-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, and Miroslav Blumenberg

8. Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline

Raphaela Rid, Harald Hundsberger, and Kamil OEnder

9. Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity

Lakiea J. Bailey, Vivek Choudhary, Purnima Merai, and Wendy B. Bollag

10. Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes

Kathleen P. McGuinn and My G. Mahoney

11. MMP-2, 9 and TIMP-1, 2 Assays in Keratinocyte Cultures

Takashi Kobayashi

12. Reactive Oxygen Species (ROS) Protection Via Cysteine Oxidation in the Epidermal Cornified Cell Envelope

Wilbert P. Vermeij and Claude Backendorf

13. Modified Methods for Growing 3-D Skin Equivalents: An Update

Rebecca Lamb and Carrie A. Ambler

14. A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro

Yoji Okugawa and Yohei Hirai

15. Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter

Evelyne De Vuyst, Celine Charlier, Severine Giltaire, Valerie De Glas, Catherine Lambert de Rouvroit, and Yves Poumay

16. Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications

John J. Wille, Jeremy J. Burdge, and Jong Y. Park

17. Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection

Laura M. Griffin, Louis Cicchini, Tao Xu, and Dohun Pyeon

18. Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle

Christine Chevalier, Jean-Francois Nicolas, and Anne-Cecile Petit

19. Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies

Ines Sequeira, Emilie Legue, Suzanne Capgras, and Jean-Francois Nicolas

20. Isolation and Characterization of a Stem Cell Side-Population from Mouse Hair Follicles

Paula L. Miliani de Marval, Sun Hye Kim, and Marcelo L. Rodriguez Puebla

21. Multi-Scale Mathematical Modeling and Simulation of Cellular Dynamical Process

Shinji Nakaoka