Main description:

This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system. Epigenetic Methods in Neuroscience Research guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples; sensitive method for quantification of alternative methylated forms of cytosines by liquid chromatography/mass spectrometry; affinity-based detection of modified cytosines by immunohistochemistry or methylated DNA immunoprecipitation; chromatin immunoprecipitation, or ChIP; miRNA high-throughput profiling and the in situ detection of miRNA subtle expression in the brain; analysis of genes with alternative 3'UTRs; and the cite-specific delivery of chromatin-modifying drugs. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Epigenetic Methods in Neuroscience Research provides multidisciplinary epigenetic approach to study genome, and neural, plasticity that will help the reader to successfully address the challenges associated with neurodevelopmental, psychiatric, and neurodegenerative disorders.

Contents:

Preface

Series Preface

Contributors

PART I INTRODUCTION

1 Epigenetics: From Basic Biology to Chromatin Modifying Drugs and New Potential Clinical Applications

Francesco Matrisciano, Isabella Panaccione, Erbo Dong, Dennis R Grayson, and Alessandro Guidotti

2 Analysis of Brain Epigenome: A Guide To Epigenetic Methods

Nina N. Karpova

PART II ANALYSIS OF CHROMATIN REMODELING

3 Analysis of DNA Methylation by Bisulfite Reaction in Neural Cells as an Example of Orexin Neurons

Koji Hayakawa, Mitsuko Hirosawa, and Kunio Shiota

4 Protocol for The Simultaneous Analysis of Gene DNA Methylation and mRNA Expression Levels in the Rodent Brain

Juzoh Umemori and Nina N. Karpova

5 Simultaneous Quantification Of Global 5mc And 5hmc Levels In The Nervous System Using A HPLC/MS Method

Daniel L. Ross and Garrett A. Kaas

6 Protocol for Methylated DNA Immunoprecipitation (Medip) Analysis

Nina N. Karpova and Juzoh Umemori

7 Cell Type-Specific DNA Methylation Analysis in Neurons and Glia

Miki Bundo, Tadafumi Kato, and Kazuya Iwamoto

8 Immunohistochemical Detection of Oxidized Forms of 5-Methylcytosine in Embryonic and Adult Brain Tissue

Abdulkadir Abakir, Lee M. Wheldon, and Alexey Ruzov

9 Analysis of Histone Modifications in Neural Cells as an Example of Orexin Neurons

Koji Hayakawa, Chikako Yoneda, Ruiko Tani, and Kunio Shiota

10 Site-Specific Delivery of Epigenetic Modulating Drugs into the Rat Brain

Amanda Sales, Caroline Biojone, and Samia Joca

PART III ANALYSIS OF NON-CODING GENOME

11 Mammalian Genome Plasticity: Expression Analysis Of Transposable Elements

Brian B. Griffiths and Richard G. Hunter

12 Tools for Analyzing the Role Of Local Protein Synthesis in Synaptic Plasticity

Lauren L. Orefice and Baoji Xu

13 In Situ Detection Of Neuron-Specific Micrornas in Frozen Brain Tissue

Asli Silahtaroglu, Silke Herzer, and Bjoern Meister

14 Protocol For High-Throughput Mirna Profiling Of The Rat Brain

Sharon L. Hollins, Fredrick R. Walke, and Murray J. Cairns

PART IV ANALYSIS OF CIRCADIAN OSCILATIONS

15 Metabolites as Clock Hands: Estimation of Internal Body Time Using Blood Metabolomics

Hitoshi Iuchi, Rikuhiro G. Yamada, and Hiroki R. Ueda