Main description:

The aim of this book is to provide experimental protocols covering many aspects of glycobiology, glycotechnology, and chemistry: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The protocols are all self-contained descriptions of the equipment and reagents needed, followed by details of the experimental procedure.

In the post-genomic era, glycobiology is coming of age because more than half of proteins are glycosylated and the importance of sugar chains in various fields of life science research cannot be disregarded. Many scientists had not entered this area because glycobiology and glycoscience used to be considered difficult fields. This book, therefore, is presented much like a cookbook which can help scientists in fields other than glycobiology and glycoscience carry out research more easily.

Contents:

1. Glycosyltransferase Genes

Part 1. Glycosyltransferases
Part 2. Sugar-Modified Enzymes
Part 3. Transporters
Part 4. Glycosidases
Part 5. Animal Lectins

2. Functional Analyses of Sugar Chains

Part 6. Immunology
Part 7. Brains and Nerves
Part 8. Quality Control of Proteins
Part 9. Golgi and Lysosomal Diseases
Part 10. Infections
Part 11. Cancer
Part 12. Regeneration Medicine and Transplantation
Part 13. Fertilization
Part 14. Development, Differentiation, and Morphogenesis
Part 15. Muscle Dystrophy and Carbohydrate Disorders of Glycosylation
Part 16. Lifestyle-Related Diseases
Part 17. IgA Nephropathy
Part 18. Growth Factor Receptors
Part 19. Blood Groups and Blood Cells

3. Glycosyltransferase Gene KO Mice

Part 20. Glycosyltransferase Gene KO Mice

4. Infrastructures and Research Resources

Part 21. Glycosyltransferases and Related Genes, and Useful Cell Lines
Part 22. Sugar Library
Part 23. Database
Part 24. Microarray