Main description:

This book discusses a broad range of basic and advanced topics in the field of protein structure, function, folding, flexibility, and dynamics. Starting with a basic introduction to protein purification, estimation, storage, and its effect on the protein structure, function, and dynamics, it also discusses various experimental and computational structure determination approaches; the importance of molecular interactions and water in protein stability, folding and dynamics; kinetic and thermodynamic parameters associated with protein-ligand binding; single molecule techniques and their applications in studying protein folding and aggregation; protein quality control; the role of amino acid sequence in protein aggregation; muscarinic acetylcholine receptors, antimuscarinic drugs, and their clinical significances.

Further, the book explains the current understanding on the therapeutic importance of the enzyme dopamine beta hydroxylase; structural dynamics and motions in molecular motors; role of cathepsins in controlling degradation of extracellular matrix during disease states; and the important structure-function relationship of iron-binding proteins, ferritins. Overall, the book is an important guide and a comprehensive resource for understanding protein structure, function, dynamics, and interaction.

Contents:

Chapter 1_ Protein purification, estimation, storage and their effect on structure, function and dynamics.- Chapter 2_Molecular motor: Subdomain dynamics and mechanochemistry.- Chapter 3_Computational insights into the role of SNPs/mutations in enzyme inhibition: progress and challenges.-Chapter 4_Use of group specific reagents in active site functional group elucidation.- Chapter 5_Understanding the potential role of structurally distant amino acids in modulating the structure and activity of proteins.- Chapter 6_Role of human genetic variations in the modulation of enzyme efficacy and activity of metabolic and xenobiotic metabolizing enzymes.- Chatper 7_Computational protein structure modeling: The gateway for drug design.- Chapter 8_Molecular dynamics simulation study of protein and protein-ligand complexes.- Chapter 9_Protein-protein interactions modeling: from wet to dry lab approaches