Main description:

The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology.

Contents:

Part A: Establishment of hairy root cultures and / or native production of Secondary Metabolites
1. Establishment of hairy root cultures for the production of Tropane alkaloids2. Establishment of hairy root cultures for the production of (mono / di / sesqui) Terpene alkaloids3. Hairy root cultures for saponins / triterpenoid saponins4. Production of of Benzene isoquinolidine / pyrolizidine alkaloids from Hairy root cultures5. Establishment of hairy root cultures for the production of Phenolics (flavonoids / quinones) compounds
Part B: Production strategies: Case studies of hairy root cultures for enhanced metabolite production

6. Optimization of culture conditions for enhanced metabolite production through hairy root cultures7. Influence of elicitation (physical / chemical/ biotic) on metabolite production in hairy root cultures8. Improvement of metabolite production in hairy roots using Metabolic engineering / pathway modulation9. Up scaling of hairy root cultures in bioreactors for enhanced biomass and proportionate metabolite production
Part C: Hairy roots as a tool for additional value applications

10. Production of valuable molecules through bioconversion of exogenous substrate using hairy root cultures

11. Utilization of hairy root cultures for Antibody / vaccine production

12. Potential of hairy root cultures for the Remediation of toxic compounds

Part D: Application of hairy root research: emerging trends

13. Mathematical / computational simulation in growth of hairy root cultures

14. Involvement of Crisper/Cas9 for genome editing in hairy root cultures

15. Functional characterization in hairy root cultures (plant-microbe interaction)

16. Agrobacterium rhizogenes mediated hairy root cultures of parasite plants to study host- parasite plant relationship