Main description:

This detailed volume encompasses chapters from leading
experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional
production and stabilization of recombinant integral membrane proteins (IMPs). Membrane
proteins play a key role in numerous pathologies such as cancer, cystic
fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on
these and other disorders are hampered by a lack of information about the
proteins involved. This book sets out to aid researchers in rectifying this
situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions
to their respective topics, lists of the necessary materials and reagents,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.

Authoritative and up-to-date, Heterologous
Expression of Membrane Proteins: Methods and Protocols, Second Edition
serves as an ideal guide for scientists attempting to delve deeper into the myriad
unique IMP structures.

Contents:

1. Cell-Free Production of Membrane
Proteins in Escherichia coli Lysates
for Functional and Structural Studies

Ralf-Bernhardt Rues, Erik Henrich,
Coilin Boland, Martin Caffrey, and Frank Bernhard

2. Membrane Protein Production in the
Yeast, S. cerevisiae

Stephanie P. Cartwright, Lina
Mikaliunaite, and Roslyn M. Bill

3. Membrane Proteins Production in Escherichia coli: Protocols and Rules

Federica Angius, Oana Ilioaia, Marc Uzan, and Bruno Miroux

4. Codon Optimizing for Increased
Membrane Protein Production: A Minimalist Approach

Kiavash Mirzadeh, Stephen Toddo,
Morten H.H. Norholm, and Daniel O. Daley

5. Generation of Tetracycline-Inducible
Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane
Proteins

Juni Andrell, Patricia C. Edwards,
Fan Zhang, Maria Daly, and Christopher G. Tate

6. Membrane Protein Production in Lactococcus lactis for Functional Studies

Daphne
Seigneurin-Berny, Martin S. King, Emiline Sautron, Lucas Moyet, Patrice Catty,
Francois Andre, Norbert Rolland, Edmund R.S. Kunji, and Annie Frelet-Barrand

7. Expression of
Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells

Renato Mancini Astray, Sandra Fernanda Suarez-Patino, and Soraia Attie
Calil Jorge

8. Leishmania
tarentolae as a Promising Tool for Expressing Polytopic and
Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC
Pump ABCG6

Lucia Gonzalez-Lobato, Vincent
Chaptal, Jennifer Molle, and Pierre Falson

9. Overexpression, Membrane Preparation,
and Purification of a Typical Multidrug ABC Transporter BmrA

Benjamin Wiseman and Jean-Michel Jault

10. Expression of
Eukaryotic Membrane Proteins in Pichia
pastoris

Lucie Hartmann, Valerie Kugler, and Renaud Wagner

11. Integral Membrane Protein Expression in Saccharomyces cerevisiae

Rebba C. Boswell-Casteel, Jennifer
M. Johnson, Robert M. Stroud, and Franklin A. Hays

12. High-Throughput Baculovirus
Expression System for Membrane Protein Production

Ravi C. Kalathur, Marinela Panganiban,
and Renato Bruni

13. Small-Scale Screening to Large-Scale
Over-Expression of Human Membrane Proteins for Structural Studies

Sarika Chaudhary, Sukanya Saha,
Sobrahani Thamminana, and Robert M. Stroud

14. Purification of Human and Mammalian
Membrane Proteins Expressed in Xenopus
laevis Frog Oocytes for Structural Studies

Rajendra Boggavarapu, Stephan
Hirschi, Daniel Harder, Marcel Meury, Zoehre Ucurum, Marc J. Bergeron, and
Dimitrios Fotiadis

15.

16. Detergent-Free Membrane Protein Purification

Alice J. Rothnie

17. Conformational Dynamics and
Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry

Eric Forest and Petr Man

18. Lessons from an a-Helical Membrane Enzyme: Expression, Purification,
and Detergent Optimization for Biophysical and Structural Characterization

Jennifer L. Johnson, Sibel Kalyoncu,
and Raquel L. Lieberman

19. Method to Screen Multidrug Transport
Inhibitors Using Yeast Over-Expressing a Human MDR Transporter

Laura Fiorini and Isabelle Mus-Veteau