Main description:

This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assays, and computational techniques to analyze HTS data are all examined. Written for the highly successful Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, High Throughput Screening Assays in Toxicology serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailing animal models.

Contents:

Part I: In Vitro Toxicological High-Thoughput Screening Methods

1. Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay

Carlos Coriano, Emily Powell, and Wei Xu

2. Mitochondrial Membrane Potential Assay

Srilatha Sakamuru, Matias S. Attene-Ramos, and Menghang Xia

3. Using -Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway

Thai Khuc, Chia-Wen (Amy) Hsu, Srilatha Sakamuru, and Menghang Xia

4. Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators

Caitlin Lynch, Jinghua Zhao, Hongbing Wang, and Menghang Xia

5. Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity

Chia-Wen (Amy) Hsu, Jinghua Zhao, and Menghang Xia

6. Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway

Jinghua Zhao, Sunita J. Shukla, and Menghang Xia

7. Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells

Yitong Liu

8. Determination of Histone H2AX Phosphorylation in DT40 Cells

Kana Nishihara, Sampada A. Shahane, and Menghang Xia

9. High-Throughput and High Content Micronucleus Assay in CHO-K1 Cells

Sampada A. Shahane, Kana Nishihara, and Menghang Xia

Part II: In Vivo Toxicological High-Thoughput Screening Methods

10. Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish

Lisa Truong, Michael T. Simonich, and Robert L. Tanguay

11. Fast Functional Germline and Epigenetic Assays in the Nematode Caenorhabditis elegans

Zachary Lundby, Jessica Camacho, and Patrick Allard

Part III: In Silico High-Thoughput Screening Toxcity Data Analysis

12. A Quantitative High-Throughput Screening Data Analysis Pipeline for Activity Profiling

Ruili Huang

13. Correction of Microplate Data from High-Throughput Screening

Yuhong Wang and Ruili Huang

14. CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprints

Alexander Sedykh

15. Accounting Artifacts in High-Throughput Toxicity Assays

Jui-Hua Hsieh

16. Accessing the High-Throughput Screening Data Landscape

Daniel P. Russo and Hao Zhu

17. Curating and Preparing High Throughput Screening Data for Quantitative Structure Activity Relationship Modeling

Marlene T. Kim, Wenyi Wang, Alexander Sedykh, and Hao Zhu