Main description:

Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.

Contents:

Part I: Infection Models to Study Bacterial Virulence

1. Galleria mellonella as an Infection Model for Select Agents

Nicolas Sprynski, Eric Valade, and Fabienne Neulat-Ripoll

2. Drosophila as a Model for Intestinal Infections

Matthieu Lestradet, Kwang-Zin Lee, and Dominique Ferrandon

3. Zebrafish Embryos as a Model to Study Bacterial Virulence

Jennifer Mesureur and Annette C. Vergunst

4. Studying Host-pathogen Interaction Events in Living Mice Visualized in Real Time using Bio photonic Imaging

Gary Splitter, Jerome Harms, Erik Petersen, Diogo Magnani, Marina Durward, Gireesh Rajashekara, and Girish Radhakrishnan

5. Intravital Two photon Imaging to Understand Bacterial Infections of the Mammalian Host

Ferdinand X. Choong and Agneta Richter-Dahlfors

PartII: Cellular Aspects of Host-Bacteria Interactions

6. Cre Reporter Assay for Translocation (CRAfT), A Tool for the Study of Protein Translocation into Host Cells

Amke den Dulk-Ras, Annette Vergunst, and Paul J.J. Hooykaas

7. Detection of the Interaction Between Host and Bacterial Proteins: Eukaryotic Nucleolin Interacts with Francisella Elongation Factor Tu

Monique Barel and Alain Charbit

8. Hijacking the Host Proteasome for the Temporal Degradation of Bacterial Effectors

Tomoko Kubori, Andree M. Hubber, and Hiroki Nagai

9. Life Cell Imaging of Phosphoinositide Dynamics during Legionella Infection

Stephen Weber and Hubert Hilbi

10. Investigating Interference with Apoptosis Induction by Bacterial Proteins

Hua Niu and Yasuko Rikihisa

11. Bimolecular Fluorescence Complementation for Imaging Protein Interactions in Plant Hosts of Microbial Pathogens

Lan-Ying Lee and Stanton B. Gelvin

12. Investigating TLR Signalling Responses in Murine Dendritic Cells upon Bacterial Infection

Suzana Pinto Salcedo and Lena Alexopoulou

Part III: OMICS and Large Scale Screening13. siRNA Screens using Drosophila Cells to Identify Host Factors Required for InfectionAseem Pandey, Sheng Li Ding, Thomas A. Ficht, and Paul de Figueiredo

14. Purification of Intracellular Bacteria: Isolation of Viable Brucella abortus from Host Cells

Esteban Chaves-Olarte, Pamela Altamirano-Silva, Caterina Guzman-Verri, and Edgardo Moreno

15. RNA Sequencing of FACS-sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens

Julien Rougeot, Ania Zakrzewska, Zakia Kanwal, Hans J. Jansen, Herman P. Spaink, Annemarie H. Meijer

16. Taking the Shortcut for High-throughput Shotgun Proteomic Analysis of Bacteria

Erica Marie Hartmann, Francois Allain, Jean-Charles Gaillard, Olivier Pible, and Jean Armengaud

17. Comparative Genomic Analysis at the PATRIC, a Bioinformatic Resource Center

Alice R. Wattam, Joseph L. Gabbard, Maulik Shukla, and Bruno W. Sobral

Part IV: Approaches for Difficult Bacteria

18. A Markerless Deletion Method for Genetic Manipulation of Burkholderia cenocepacia and

Other Multi Antibiotic Resistant Gram-negative Bacteria

Daniel F. Aubert, Mohamad A. Hamad, and Miguel A. Valvano

19. Gene Inactivation in Coxiella burnetii

Paul A. Beare and Robert A. Heinzen

20. A Chemical Mutagenesis Approach to Identify Virulence Determinants in the Obligate Intracellular Pathogen Chlamydia trachomatis

Bidong Nguyen and Raphael Valdivia