Main description:

Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world. The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies. Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.

Contents:

Part I: Maintenance and Cryopreservation of ES and iPS cells

1. Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells

Dong-Youn Hwang

2. Growth of Human Embryonic Stem Cells in Long-Term Hypoxia

Vladimir Zachar, Simon C. Weli, Mayuri S. Prasad, and Trine Fink

3. Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein that Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells

Masato Nagaoka and Stephen A. Duncan

4. Scale-Up of Single Cell-Inoculated Suspension Cultures of Human Embryonic Stem Cells

Harmeet Singh, Pamela Mok, and Robert Zweigerdt

5. Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells

Guang-wei Sun, Xiao-xi Xu, Nan Li, Ying Zhang, and Xiao-jun Ma

6. Expansion of Pluripotent Stem Cells in Defined Xeno-Free Culture System

Kristiina Rajala

7. Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells

Behrouz Aflatoonian and Harry D. Moore

8. Cryopreservation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Frida Holm

Part II: Generation of Human Induced Pluripotent Stem Cells: Viral and Nonviral Vector-Based Nuclear Reprogramming

9. Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2

Alessandra Giorgetti, Nuria Montserrat, and Juan Carlos Izpisua Belmonte

10. Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood

Naoki Nishishita, Chiemi Takenaka, and Shin Kawamata

11. Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and Their Differentiation to Neuronal Lineage

Maria V. Shutova, Ilya V. Chestkov, Maria A. Lagarkova, and Sergey L. Kiselev

12. Generation of Human Induced Pluripotent Stem Cells from Endoderm Origin Cells

Hua Liu, Su Mi Choi, and Yoon-Young Jang

13. Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders

Kazutoshi Takahashi

14. Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)

Hiroe Ohnishi, Yasuaki Oda, and Hajime Ohgushi

15. Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from Fibroblasts and Keratinocytes

Athanasia D. Panopoulos, Sergio Ruiz, and Juan Carlos Izpisua Belmonte

16. Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Follicles

Ann Peters and Elias T. Zambidis

Part III: Generation of Patient-Specific iPS Cells for Clinical Application

17. Generation of iPS Cells from Human Skin Biopsy

Katie Avery and Stuart Avery

18. Generation of Induced Pluripotent Stem Cells from Human Amnion Cells

Masashi Toyoda, Shogo Nagata, Hatsune Makino, Hidenori Akutsu, Takashi Tada, and Akihiro Umezawa

Part IV: Lineage-Specific Differentiation of hES and iPS Cells

19. In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells

Xiaolong Lin, Hua Jiang, Zack Zhengyu Wang, and Tong Chen

20. Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells

Kumiko Saeki

21. Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endothelial Cells under Hypoxic Condition

Xiuli Wang

22. Differentiation of Endothelial Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Shijun Hu, Preston Lavinghousez, Zongjin Li, and Joseph C. Wu

23. Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Blood Cells in Coculture with Murine Stromal Cells

Feng Ma, Yanzheng Gu, Natsumi Nishihama, Wenyu Yang, Ebihara Yasuhiro, and Kohichiro Tsuji

24. Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells

Tea Soon Park, Paul W. Burridge, and Elias T. Zambidi

25. Adipogenic Differentiation of Human Induced Pluripotent Stem Cells

Michio Noguchi, Masakatsu Sone, Daisuke Taura, Kiminori Hosoda, and Kazuwa Nakao

26. Chondrogenic Differentiation of hESC in Micromass Culture

Deborah Ferrari, Guochun Gong, Robert A. Kosher, and Caroline N. Dealy

27. Deriving Hepatic Endoderm from Pluripotent Stem Cells

Claire N. Medine, Zara Hannoun, Sebastian Greenhough, Catherine M. Payne, Judy Fletcher, and David C. Hay

28. Multistage Hepatic Differentiation from Human Induced Pluripotent Stem Cells

Su Mi Choi, Hua Liu, Yonghak Kim, and Yoon-Young Jang

29. Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers

Takamichi Ishii and Kentaro Yasuchika

30. Generation of Lung Epithelial-Like Tissue from hESC by Air-Liquid Interface Culture

Lindsey Van Haute, Gert De Block, Inge Liebaers, Karen Sermon, and Martine De Rycke

31. Direct Differentiation of Human Embryonic Stem Cells into Selective Neurons on Nanoscale Ridge/Groove Pattern Arrays

Kye-Seong Kim, Hosup Jung, and Keesung Kim

Part V: Directed Differentiation of hES and iPS Cells in 3D Environments

32. Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and MatrigelTM Gels

Eric Derby, Dezhong Yin, Wei-Qiang Gao, and Wu Ma

Part VI: Qualitative and Quantitative Analysis of Dynamics of hES Cell Differentiation

33. Single-Cell Transcript Profiling of Differentiating Embryonic Stem Cells

Jason D. Gibson, Caroline M. Jakuba, Craig E. Nelson, and Mark G. Carter

34. Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripotent Stem Cells

Agnete Kirkeby, Malin Parmar, and Johan Jakobsson