Main description:

Human Fertility: Methods and Protocols is intended for all practitioners of reproductive medicine and ART, as well as for embryologists and reproductive, developmental, cell and molecular biologists and others in the biomedical sciences. The volume presents straight-forward manner best practice approaches for overcoming a host of fertility challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Human Fertility: Methods and Protocols aids scientists in continuing to study assisted reproductive technologies.

Contents:

Part I: Overview

1. General Aspects of Fertility and Infertility

Mark A. Damario

2. Genetics of Male Fertility

Yi-Nan Lin and Martin M. Matzuk

3. Genetics of Female Infertility due to Anomalies of the Ovary and Mullerian Ducts

Joe Leigh Simpson

4. Gene Polymorphisms in Female Reproduction

Livio Casarini, Manuela Simoni

5. Understanding the Spermatozoon

Queenie V. Neri, Jennifer Hu, Zev Rosenwaks, and Gianpiero D. Palermo

6. Derivation of Human Embryonic Stem Cells (hESC)

Nikica Zaninovic, Qiansheng Zhan, and Zev Rosenwaks

7. The Endocrinology of the Menstrual Cycle

Robert L. Barbieri

8. Assisted Reproductive Techniques

Jack Yu Jen Huang and Zev Rosenwaks

9. Novel Markers of Male Infertility

Michael Funaro and Darius A. Paduch

10. Luteal Phase Support in ART Treatments

Yuval Or, Edi Vaisbuch, and Zeev Shoham

11. General Principles of Cryopreservation

Roger G. Gosden

Part II: Methodology

12. In Vitro Maturation of Immature Human Oocytes for Clinical Application

Ri-Cheng Chian and Yun-Xia Cao

13. GnRH Antagonist Based Protocols for In Vitro Fertilization

David Reichman and Zev Rosenwaks

14. Ovarian Stimulation for IVF: Mild Approaches

O. Hamdin, F.J. Broekmans, B.C.J.M. Fauser

15. IVF Stimulation: Protocols for Poor Responders

Owen K. Davis

16. Oocyte Retrieval and Quality Evaluation

Lucinda Veeck Gosden

17. Sperm Retrieval and Quality Evaluation

Peter J. Stahl, Peter N. Schlegel, and Marc Goldstein

18. Treatment of Male Infertility

Gianpiero D. Palermo, Justin Kocent, Devin Monahan, Queenie V. Neri, and Zev Rosenwaks

19. Techniques for Slow Cryopreservation of Embryos

Lucinda Veeck Gosden

20. Cryopreservation of Eggs

Peter Nagy

21. Ovarian Tissue Cryopreservation and Transplantation. A Realistic, Effective Technology for Fertility Preservation

Dror Meirow, Hila Ra'anani, and Hannah Biderman

22. Detection of Monogenic Disorders and Chromosome Aberrations by Preimplantation Genetic Diagnosis

Kangpu Xu and David Reichman

23. Embryo Culture and Selection: Morphological Criteria

Aparna Hegde and Barry Behr

24. Embryo Selection using Metabolomics

D. Sakkas

25. Embryo Transfer

Anate Aelion Brauer and Glenn Schattman

26. Safety of Assisted Reproductive Technologies

Gianpiero D. Palermo, Queenie V. Neri, and Zev Rosenwaks

27. Human Germ Cell Differentiation from Pluripotent Embryonic Stem Cells and Induced Pluripotent Stem Cells

Jose V Medrano, Carlos Simon, and Renee Reijo Pera