Main description:

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.

Contents:

The Biological Chemistry of Hydrogen Peroxide

Reactive Oxygen Species in the Activation of MAP Kinases

Hydrogen Peroxide Signaling Mediator in the Activation of p38 MAPK in Vascular Endothelial Cells

In vivo Imaging of Nitric Oxide and Hydrogen Peroxide in Cardiac Myocytes

Methods for Studying Oxidative Regulation of Protein Kinase C

P66Shc, Mitochondria and the Generation of Reactive Oxygen Species

Detecting Disulfide Bound Complexes and the Oxidative Regulation of Cyclic Nucleotide Dependent Protein Kinases by H2O2

Redox Regulation of Protein Tyrosine Phosphatases: Methods for Kinetic Analysis of Covalent Enzyme Inactivation

Activation of Nrf2 by H2O2: De novo Synthesis versus Nuclear Translocation

H2O2 in the Induction of NF-k B-Dependent Selective Gene Expression

Detection of H2O2-Mediated Phosphorylation of Kinase-Inactive PDGFRa

Genetic Modifier Screens to Identify Components of a Redox-Regulated Cell Adhesion and Migration Pathway

Investigating the Role of Reactive Oxygen Species in Regulating Autophagy

H2O2: A Chemoattractant?

Measuring Mitochondrial Uncoupling Protein-2 Level and Activity in Insulinoma Cells

Effects of H2O2 on Insulin Signaling and the Glucose Transport System in Mammalian Skeletal Muscle

Monitoring of Hydrogen Peroxide and Other Reactive Oxygen and Nitrogen Species Generated by Skeletal Muscle