Main description:

This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Contents:

Part A: Techniques

Laser Induced Radiation Microbeam Technology and Simultaneous Real-time Fluorescence Imaging in Live Cells
A cell biologist's guide to high resolution imaging
Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo
Detecting and Tracking Non-Fluorescent Nanoparticle Probes in Live Cells
Fluorescence lifetime microscopy of tumor cell invasion, drug delivery and cytotoxicity
Measuring membrane protein dynamics in neurons using Fluorescence recovery after photobleach (FRAP)
Distinctive characteristics of Madeira wine regarding its traditional winemaking and modern analytical methodologies
Green to red photo-convertible mEosFP aided live imaging in plants
Methods for Cell and Particle Tracking
Correlative light-electron microscopy: a potent tool for the imaging of rare or unique cellular and tissue events and structures
Optical Techniques for Imaging Membrane Domains in Live Cells (Live Cell Palm of Protein Clustering)
Single live cell topography and activity imaging with the Shear-Force-Based constant-distance scanning electrochemical microscope
Visualization of TGN-endosome trafficking in mammalian and drosophila cells
Live cell imaging with chemical specificity using dual frequency CARS microscopy
Imaging intracellular protein dynamics by spinning disk confocal microscopy

Part B: Tools

Visualizing Dynamic Activities of Signaling Enzymes using genetically encodable FRET-based biosensors: from designs to applications
Live-cell Imaging of Aquaporin-4 Supramolecular Assembly and Diffusion
Coiled-coil tag-probe labeling methods for live-cell imaging of membrane receptors
Monitoring Protein Interactions in Living Cells with Fluorescence Lifetime Imaging Microscopy
Open Source Tools for Fluorescent Imaging
Nanoparticle PEBBLE sensors in live cells