Main description:

This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Contents:

Part A: Viruses, Microbes and Parasites

Nanoscale imaging of microbial pathogens using atomic force microscopy

Challenges in imaging parasites

Part B: Disease States Real-time live imaging to study bacterial infections in vivo

Analysis of cellular membrane dynamics by following virus endocytosis

Imaging of live malaria blood stage parasites

Escherichia coli K1 invasion of human brain microvascular endothelial cells

Real-time imaging after cerebral ischemia

Imaging acute inflammation in zebrafish

Imaging protein oligomerization in neurodegeneration using BiEC

Imagng and quantifying the dyanmics of tumor-associated proteolysis

Part C: Other Techniques

Live cell imaging in vivo with fluorescent proteins

Visualization of gene expression

Imaging stem cell implant for cellular-based therapies

Live cell single molecule imaging to investigate early events in B lymphocyte signaling

A quantitative method for measuring phototoxicity of a live cell imaging microscope

High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines

High-content screening of primary neurons

Live-imaging fluorescent proteins in early mouse embryos

Comparing the methods for quantitative analysis of dendritic spine dynamics

Live-imaging of cell competition in drosophila imaginal epithelium

Live cell imaging of the oviduct

Computational estimation of leukocyte numbers in zebrafish

Four dimensional tracking of lymphocyte migration and interactions by two-photon microscopy