Main description:

This fully updated book brings together protocols to arm stem cell biologists with tools and approaches to continue uncovering the intricacies and regulatory mechanisms underlying stem cell biology. Through various models and organ systems, the volume reflects the numerous recent advances in cell lineage and lineage tracking. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Imaging and Tracking Stem Cells: Methods and Protocols, Second Edition is an ideal guide for novices and experts alike who are working to expand our knowledge in the field of stem cells.

Contents:

1. Molecular Imaging and Tracking Stem Cells in Neurosciences

Nora Sandu, Thomas Rosemann, and Bernhard Schaller

2. Tracking Germline Stem Cell Dynamics In Vivo in C. elegans Using Photoconversion

Simona Rosu and Orna Cohen-Fix

3. Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy

Ina Maria Schiessl, Katharina Fremter, James L. Burford, Hayo Castrop, and Janos Peti-Peterdi

4. Tracking and Imaging of Transplanted Stem Cells in Animals

Tanmayee Rajeev Chikate and Liping Tang

5. Generation and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes and Endothelial Cells for High Content Screening Purposes

Tunde Berecz, Maria Husveth-Toth, Maxime Mioulane, Bela Merkely, Agota Apati, and Gabor Foeldes

6. Covisualization of Global DNA Methylation/Hydroxymethylation and Protein Biomarkers for Ultra-High Definition Epigenetic Phenotyping of Stem Cells

Jian Tajbakhsh

7. A Distinctive MRI-Based Absolute Bias Correction Protocol for the Potential Labeling and In Vivo Tracking of Stem Cells in a TBI Mice Model

Sushanta Kumar Mishra, Subash Khushu, and Gurudutta Gangenahalli

8. Efficient Labeling of Human Mesenchymal Stem Cells Using Iron Oxide Nanoparticles

Sonali Rawat, Suchi Gupta, Madhusudan Bhat, Amit Kumar Dinda, and Sujata Mohanty

9. Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling

Xuan Su, Yan Shen, Neal L. Weintraub, and Yaoliang Tang

10. Long-Term Intravital Imaging of Cornea, Skin, and Hair Follicle by Multiphoton Microscope

Yueh-Feng Wu, Hsin-Yuan Tan, and Sung-Jan Lin

11. Cell Cycle Analysis Using In Vivo Staining of DNA Synthesizing Cells

Petr Paral, Martin Bajecny, Filipp Savvulidi, and Emanuel Necas

12. Metabolic Labeling of Live-Stem Cell for In Vitro Imaging and In Vivo Tracking

Sang-Soo Han and Sun-Woong Kang

13. Study of Intracellular Cargo Trafficking and Co-Localization in the Phagosome and Autophagy-Lysosomal Pathways of Retinal Pigment Epithelium (RPE) Cells

J. Arjuna Ratnayaka, Eloise Keeling, and David S. Chatelet

14. Timelapse Video-Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro

Lucia Paniagua-Herranz, Rosa Gomez-Villafuertes, David de Agustin-Duran, Sergio Gascon, Raquel Perez-Sen, Esmerilda G. Delicado, Maria Teresa Miras-Portugal, and Felipe Ortega

15. Multiphoton Microscopy for Non-Invasive and Label-Free Imaging of Human Skin and Oral Mucosa Equivalents

Gopu Sriram, Sudhaharan Thankiah, and Graham D. Wright

16. Molecular Imaging of Therapeutic Effect of Mesenchymal Stem Cell-Derived Exosomes for Hindlimb Ischemia Treatment

Kaiyue Zhang and Zongjin Li

17. In Vitro Methods to Simulate Pollution and Photo-Pollution Exposure in Human Skin-Epidermis

Namita Misra and Laurent Marrot