Main description:

This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basics to the most complex overview of method and protocols. There is ample practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters, licensed software and a unique, continuously updated website forum.

Contents:

Considerations for Routine Imaging.- Entering the Portal: Understanding the Digital Image Recorded Through a Microscope.- Quantitative Biological Image Analysis.- The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics.- Design and Function of a Light-Microscopy Facility.- Advanced Methods and Concepts.- Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls.- Quantitative FRET Microscopy of Live Cells.- Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells.- Single Fluorescent Molecule Tracking in Live Cells.- From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins.- Cutting-Edge Applications and Utilities.- Towards Imaging the Dynamics of Protein Signalling.- New Technologies for Imaging and Analysis of Individual Microbial Cells.- Imaging Parasites in Vivo.- Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells.- High-Throughput/High-Content Automated Image Acquisition and Analysis.- Cognition Network Technology - A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents.- High-Content Phenotypic Cell-Based Assays.