Main description:

Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments:

* producing transgenic animals

* studying the effects of drug treatments

* electrophysiological studies

* animal surgery * isolating and differentiating stem cells

* super-resolution microscopy

Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.

Contents:

Short Historic Overview.- Primary Antibodies.- Choosing Commercial Suppliers of Primary Antibodies.- Detection and Visualization of Antigens.- Ancillary Reagents for IHC.- Preparing Tissues for IHC Staining.- Staining Protocols.- Microscopy Examination and Collection of Digital Images and Their Presentation.- Concluding Remarks.