Main description:

Recent critical advances in the field of immunotoxicology have led to incredible contributions to basic science, including crucial improvements in human risk assessment. In Immunotoxicity Testing: Methods and Protocols, expert researchers explore these changes, providing the reader with current, lab-ready procedures, along with the corresponding background information that is necessary to identify effective testing approaches for chemicals and drugs. Among the first volumes to meld consideration of immunotoxicity testing strategies with a comprehensive presentation of detailed laboratory protocols, chapters include a description of the evolution of immunotoxicity testing, a look at the importance of immunotoxicity testing for health risk reduction, and ideas concerning the future of the field. Composed in the highly successful Methods in Molecular Biology(tm) series format, most chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips from the experts on troubleshooting and avoiding known pitfalls.

Comprehensive and innovative, Immunotoxicity Testing: Methods and Protocols is a critical, one-stop reference resource for the most important and commonly used laboratory protocols in immunotoxicology.

Contents:

Immunotoxicity Testing: Methods and Protocols
Editor Rodney R. Dietert

Table of Contents:

Table of Contents

Preface

Contributors

Part I - Introduction

Immunotoxicology Testing: Past and Future

Michael I. Luster and G. Frank Gerberick

Part II - Overview and Health-Risk Considerations

Developmental Immunotoxicity (DIT): The Why, When and How of DIT Testing.

Rodney R. Dietert and Jamie DeWitt

An In Vivo Approach to Test Immunosensitization by Low Moelcular Weight Compounds

Irene S. Ludwig, Lydia M. Kwast, Danielle Fiechter, Raymond H.H. Pieters

Risk of Autoimmune Disease: Challenges for Immunotoxicity Testing

Rodney R. Dietert, Janice M. Dietert and Jerrie Gavalchin

Markers of Inflammation

Dori R. Germolec, Rachel P. Frawley, and Ellen Evans

Evaluating Macrophages in Immunotoxicity Testing

John B. Barnett and Kathleen M. Brundage

Part III - Immunotoxicity and Host Resistance Models

Host Resistance Assays Including Bacterial Challenge

Florence G. Burleson and Gary R. Burleson

Viral Host Resistance Studies

Wendy Jo Freebern

Parasite Challenge as Host Resistance Models for Immunotoxicity Testing

Robert W. Luebke

Tumor Challenges in Immunotoxicity Testing

Sheung Ng, Kotaro Yoshida, and Judith T. Zelikoff

Part IV - Testing Protocols inRodents and Other Laboratory Animals

The T-dependent Antibody Response to Keyhole Limpet Hemocyanin in Rodents

Lisa M. Plitnick and Danuta J. Herzyk

The T-Dependent Antibody Response to Sheep Erythrocytes

Kimber L. White, Jr., Deborah L. Musgrove, and Ronnetta D. Brown

13. The Delayed Hypersensitivity (DTH) Assay Using Protein and Xenogeneic Cell Antigens
Rodney R. Dietert, Terry L. Bunn and Ji-Eun Lee

14. The Cytotoxic T Lymphocyte (CTL) Assay For Evaluating Cell-Mediated Immune Function
Gary R. Burleson, Florence G. Burleson and Rodney R. Dietert
15. NK Cell Assays in Immunotoxicity Testing
Qing Li

16. The Local Lymph Node Assay and Skin Sensitization Test
Ian Kimber and Rebecca J. Dearman
17. Use of Contact Hypersensitivity in Immunotoxicity Testing
Jacques Descotes

18. Evaluation of Apoptosis in Immunotoxicity Testing
Sadiye Amcaoglu Rieder, Dilip Vakharia, Prakash S. Nagarkatti
and Mitzi Nagarkatti

19. Dendritic Cells in Immunotoxicity Testing
Donghong Gao and David A. Lawrence

20. Evaluating Cytokines in Immunotoxicity Testing
Emanuela Corsini an