Main description:

The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.

Contents:

1. Assessing Cancer Cell Migration And Metastatic Growth In Vivo In The Chick Embryo Using Fluorescence Intravital Imaging

Hon Sing Leong, Ann F. Chambers, and John D. Lewis

2. The Use Of Fluorescent Proteins For Intravital Imaging Of Tumor Cell Invasion

James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg, Jeffrey Wyckoff, John Condeelis, and Jeffrey E. Segall

3. High Resolution In Vivo Imaging Of Fluorescent Proteins Using Window Chamber Models

Gregory M. Palmer, Andrew N. Fontanella, Siqing Shan, and Mark W. Dewhirst

4. In Vivo Imaging of Pancreatic Cancer with Fluorescent Proteins in Mouse Models

Michael Bouvet and Robert M. Hoffman

5. Lentivirus-Based DsRed-2-Transfected Pancreatic Cancer Cells for Deep In Vivo Imaging of Metastatic Disease

Zeqian Yu, Jiahua Zhou, and Robert M. Hoffman

6. Non-Invasive And Real-Time Fluorescence Imaging of Peritoneal Metastasis In Nude Mice

Hayao Nakanishi, Seiji Ito, Makoto Matsui, Yuichi Ito, Kazunari Misawa, Yasuhiro Kodera

7. Three-Dimensional In Vivo Imaging Of Tumors Expressing Red Fluorescent Proteins

Alexander P. Savitsky, Irina G. Meerovich, Viktoria V. Zherdeva, Lyaisan R. Arslanbaeva, Olga S. Burova, D.V. Sokolova. E.M. Treshchalina, Anatoly Y. Baryshnikov, Ilya I. Fiks, Anna G. Orlova, Mikhail S. Kleshnin, Ilya V. Turchin, and Alexander M. Sergeev

8. Real Time Visualization and Characterization of Tumor Angiogenesis and Vascular Response to Anti-Cancer Therapies

H. Rosie Xing and Qingbei Zhang

9. In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus

Toshiyoshi Fujiwara

10. Real-Time Fluorescent Imaging of Abdominal, Pleural, and Lymphatic Metastases

Susanne Carpenter and Yuman Fong

11. Real-Time Imaging Of Tumors Using Replication-Competent Light-Emitting Microorganisms

Yong A. Yu, Stephanie Weibel, Aladar A. Szalay

12. GFP-Transgenic Animals For In Vivo Imaging: Rats, Rabbits And Pigs

Takashi Murakami and Eiji Kobayashi

13. The Use Of Fluorescent Proteins For Developing Cancer-Specific Target Imaging Probes

Thomas McCann, Nobuyuki Kosaka, Peter L. Choyke, and Hisataka Kobayashi

14. In Vivo Imaging Of The Developing Mouse Embryonic Vasculature

Irina V. Larina and Mary E. Dickinson

15. Screening Reef Corals For Novel GFP-Type Fluorescent Proteins By Confocal Imaging

Anya Salih

16. What Does It Take To Improve Existing Fluorescent Proteins For In Vivo Imaging Applications?

Marc Zimmer

17. In Vivo Imaging of Oligonucleotide Delivery

Fumitaka Takeshita, Ryou-u Takahashi, Jun Onodera, and Takahiro Ochiya

18. Subcellular Imaging In Vivo: The Next GFP Revolution

Robert M. Hoffman