Main description:

This detailed volume includes a rich variety of
applications using various instrumentations, probes, disease models, and
targets in order to account for the multidisciplinary nature of the use of in
vivo fluorescence imagine. The book also includes chapters on the emerging
fields of cell tracking, image-guided treatment, and fluorescence imaging in
the second NIR window, as well as protocols for evaluation methods before and
after in vivo imaging. Written for the highly successful Methods in Molecular Biology series, chapters include brief
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Authoritative and practical, In Vivo Fluorescence Imaging: Methods and Protocols serves as a valuable reference for researchers from numerous
fields who wish to become more familiar with in vivo fluorescence imaging
techniques.

Contents:

1. Before In Vivo
Imaging: Evaluation of Fluorescent Probes using Fluorescence Microscopy,
Multiplate Reader, and Cytotoxicity Assays

Shaojuan
Zhang

2. Bioconjugation
Methods for Coupling Targeting Ligands with Fluorescent Dyes

Xiaoxi Ling

3. In Vivo
Fluorescence Reflectance Imaging with Subcutaneous Mouse Tumor Models

Jie Cao and
Mingzhou Zhou

4. FRET Imaging of
Enzymatic Activities Using Smart Probes

Jinbo Li,
Yan Zhang, and Zhen Cheng

5. Fluorescence
Imaging of Inflammation in Live Animals

Mingzhou
Zhou, Jie Cao, and Walter J. Akers

6. Fluorescence
Lifetime Imaging of Cancer In Vivo

Owen Peng
and Walter J. Akers

7. Non-Invasive
Imaging of Fluorescent Reporters in Small Rodent Models Using Fluorescence
Molecular Tomography

Weizhou Hou
and Steve H. Thorne

8. In Vivo Tumor
Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

Rui Huang, Peter S. Conti, and Kai Chen

9. Intraoperative
Fluorescence Imaging and Multimodal Surgical Navigation Using Goggle System

Christopher
A. Mela, Francis A. Papay, and Yang Liu

10. Utilization of
Near-Infrared Fluorescent Imaging for Pharmaceutically Relevant Applications

Jelena M.
Janjic, Sravan Kumar Patel, and Christina Bagia

11. In Vivo Cell
Tracking Using Two-Photon Microscopy

Daniela
Malide

12. Small Animal
Imaging Using Diffuse Fluorescence Tomography

Scott C.
Davis and Kenneth M. Tichauer

13. In Vivo Metal Ion
Imaging Using Fluorescent Sensors

Genevieve
C. Van de Bittner and Tasuku Hirayama

14. Using
Fluorescence Imaging to Track Drug Delivery and Guide Treatment Planning In
Vivo

Qiaoya Lin, Huang Huang, Juan Chen, and
Gang Zheng

15. In Vivo
Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes

Guosong
Hong and Hongjie Dai

16. Ex Vivo Imaging,
Biodistribution, and Histological Study in Addition to In Vivo Imaging

Shaojuan
Zhang

17. Experimental
Design and Data Analysis of In Vivo Fluorescence Imaging Studies

Ying Ding
and Hui-Min Lin