Main description:

Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.

Contents:

Part I: Analysis of Viral Nucleic Acid Sensing In-Silico and In-Vitro

1. Detection of RNA Modifications by HPLC Analysis and Competitive ELISA

Gernot Nees, Andreas Kaufmann and Stefan Bauer

2. Enzymatic Synthesis and Purification of a Defined RIG-I Ligand

Marion Goldeck, Martin Schlee, Gunther Hartmann, and Veit Hornung

3. Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis

Filiz Civril and Karl-Peter Hopfner

4. Isolation of RIG-I-associated RNAs from Virus Infected Cells

Andreas Schmidt, Andreas Linder, Nicolas Linder, and Simon Rothenfusser

5. Structure Modeling of Toll-like Receptors

Jing Gong and Tiandi Wei

6. Nucleic Acid Recognition in Dendritic Cells

Alexander Heiseke, Katharina Eisenacher, and Anne Krug

7. Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems

Andrea Ribeiro and Markus Woernle

8. Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro

Simone Romoli, Adriana Migliorini

9. In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes

Saskia Ziegler and Isabelle Bekeredjian-Ding

10. Mapping of Optimal CD8 T Cell Epitopes

Julia Roider, Thomas Vollbrecht and Rika Draenert

11. A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium, and Viral Infection

Viktor H. Koelzer and David Anz

12. MicroRNA Methodology: Advances in miRNA-technologiesTheresa Kaeuferle, Sabine Bartel, Stefan Dehmel, and Susanne Krauss-Etschmann

Part II: Analysis of Nucleic Acid Sensing In-Vivo

13. Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR)

Maciej Lech and Hans-Joachim Anders

14. Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus ErythematosusAmanda A. Watkins, Ramon G. B. Bonegio and Ian R. Rifkin

15. Induction and Analysis of Nephrotoxic Serum Nephritis in Mice

John M. Hoppe and Volker Vielhauer

16. Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice

Moritz Rapp, David Anz, and Max Schnurr

17. Bifunctional siRNAs for Tumor Therapy

Fanny Matheis and Robert Besch