Main description:

This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as an ideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.

Contents:

1. Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules

Caleb B. Morris, James C. Poland, Jody C. May, and John A. McLean

Part I: Metabolomics and Lipidomics

2. Utilizing Drift Tube Ion Mobility Spectrometry for the Evaluation of Metabolites and Xenobiotics

Melanie T. Odenkirk and Erin S. Baker

3. Untargeted Differential Metabolomics Analysis Using Drift Tube Ion Mobility-Mass Spectrometry

Rick Reisdorph, Cole Michel, Kevin Quinn, Katrina Doenges, and Nichole Reisdorph

4. Drift Tube Ion Mobility-Mass Spectrometry for Non-Targeted 'Omics

Tim J. Causon, Ruwan T. Kurulugama, and Stephan Hann

5. The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines

Nicholas B. Vera, Michelle Clasquin, Stephen L. Coy, and Paul Vouros

6. Traveling Wave Ion Mobility-Mass Spectrometry to Enhance the Detection of Low Abundance Features in Untargeted Lipidomics

Zeeshan Hamid and Andrea Armirotti

7. Lipidomics by HILIC-Ion Mobility-Mass Spectrometry

Amy Li, Kelly M. Hines, and Libin Xu

8. A Workflow for the Identification of Mycotoxin Metabolites Using Liquid Chromatography-Ion Mobility-Mass Spectrometry

Laura Righetti and Chiara Dall'Asta

9. Quantitation of Cyclosporin A in Cell Culture Media by Differential Mobility Mass Spectrometry (DMS-MS/MS)

Amol Kafle, James Glick, Stephen L. Coy, and Paul Vouros

Part II: Proteomics and Glycomics

10. An Analytical Perspective on Protein Analysis and Discovery Proteomics by Ion Mobility-Mass Spectrometry

Johannes P.C. Vissers and Michael McCullagh

11. Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics

Lauren J. Tomlinson and Claire E. Eyers

12. Liquid Extraction Surface Analysis (LESA) High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Mass Spectrometry for In Situ Analysis of Intact Proteins

Rian L. Griffiths, Klaudia I. Kocurek, and Helen J. Cooper

13. Ion Mobility-Mass Spectrometry of Glycoconjugates

Weston B. Struwe and David J. Harvey

Part III: Ambient Ionization and Imaging Ion Mobility Mass Spectrometry

14. Desorption Atmospheric Pressure Photoionization Coupled with Ion Mobility-Mass Spectrometry

Tiina J. Kauppila

15. Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation

Sylwia A. Stopka and Akos Vertes

16. Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility

James I. Langridge and Emmanuelle Claude

Part IV: Bioinformatic Solutions for Analyzing IM-MS Data and Deriving CCS Values

17. The Use of LipidIMMS Analyzer for Lipid Identification in Ion Mobility-Mass Spectrometry-Based Untargeted Lipidomics

Xi Chen, Zhiwei Zhou, and Zheng-Jiang Zhu

18. MolFind2: A Protocol for Acquiring and Integrating MS3 Data to Improve In-Silico Chemical Structure Elucidation for Metabolomics

Milinda A. Samaraweera, Dennis W. Hill, and David F. Grant

19. Collision Cross Section Calculations Using HPCCS

Gabriel Heerdt, Leandro Zanotto, Paulo C.T. Souza, Guido Araujo, and Munir S. Skaf